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Plasmid#76352Purpose3rd generation lentiviral gRNA plasmid targeting human EPHB4DepositorInsertEPHB4 (EPHB4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB4 gRNA (BRDN0001148436)
Plasmid#76353Purpose3rd generation lentiviral gRNA plasmid targeting human EPHB4DepositorInsertEPHB4 (EPHB4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV TIP60 390-513
Plasmid#78797PurposeTo overexpress TIP60 390-513 in Mammalian CellsDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutationaa390-513PromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.CBX8ΔChromo
Plasmid#224708PurposeExpresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tagDepositorInsertCBX8 chromodomain truncation (CBX8 Human)
UseTagsHMBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.CBX8-KR21A
Plasmid#224709PurposeExpresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertCBX8 KR21A mt (CBX8 Human)
UseTagsHMBPExpressionInsectMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.CBX8
Plasmid#224707PurposeExpresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertCBX8 (CBX8 Human)
UseTagsHMBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt2
Plasmid#220240PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseTags6x His, MBPExpressionInsectMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt1
Plasmid#220239PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
UseTags6x His, MBPExpressionInsectMutationR27A, R31A, F165APromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
S+Q111R+N122D
Plasmid#167175PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cellsDepositorInsertsATP1A1-S+Q111R+N122D
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged Q at 111 to R and changed N at 122 to DPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+10subs
Plasmid#167176PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+10R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged A at 112 to T; E at 116 to D; I at 135 to…PromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+12subs
Plasmid#167177PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+12R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged Q at 111 to R; A at 112 to T; E at 116to …PromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-ARF5-T161A-mNeonGreen
Plasmid#162026PurposeFast cycling ARF mutantDepositorInsertARF5 (ARF5 Human)
UseLentiviralTagsExpressionMutationT161APromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.RBBP4
Plasmid#125164PurposeExpresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertHistone-binding protein RBBP4 (RBBP4 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2
Plasmid#125161PurposeExpresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertEZH2 (EZH2 Human)
UseBaculovirus expression vectorTagsHis-MBPExpressionInsectMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.AEBP2
Plasmid#125165PurposeExpresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertZinc finger protein AEBP2 (AEBP2 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EED
Plasmid#125163PurposeExpresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPolycomb protein EED (EED Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL
Plasmid#111394PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTags6xHis, Myc, T7, and XpressExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE
Plasmid#111393PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)DepositorInsertsUseAAVTags6xHis, Myc, T7, and XpressExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
CSNK1D gRNA (BRDN0001149348)
Plasmid#76716Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1DDepositorInsertCSNK1D (CSNK1D Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFB-CG-LYCHOS WT-HA-GFP
Plasmid#199687PurposeExpression of LYCHOS WT in insect cellsDepositorInsertLYCHOS (GPR155 Human)
UseTags10xHis, EGFP, HA, HRV 3C, and HisExpressionInsectMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only