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pPD548 ZF54x6-Compact_EYFP

Plasmid

#138862

Purpose

EYFP reporter vector for the ZF54x6-Compact promoter

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF54x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF54x6-Compact

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPD545 ZF37x6-Compact_EYFP

Plasmid

#138859

Purpose

EYFP reporter vector for the ZF37x6-Compact promoter

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF37x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF37x6-Compact

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD543 ZF21x6-Compact_EYFP

Plasmid

#138857

Purpose

EYFP reporter vector for the ZF21x6-Compact promoter

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF21x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF21x6-Compact

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1821

Plasmid

#163092

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mScarlet^SEC (Lox511I)^::3xMyc

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE041 CAG mKate2 (TUPV 1)

Plasmid

#138721

Purpose

mMoClo TUPV, with CAG promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

CAG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1850

Plasmid

#154328

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi5605 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI165

Plasmid

#126074

Purpose

Vector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10 (improved)

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB3038(XII-1 MarkerFree)

Plasmid

#73278

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3040(XII-4 MarkerFree)

Plasmid

#73280

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pYSD300

Plasmid

#212930

Purpose

Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA1 (AGA1 Budding Yeast)

Expression

Yeast

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-35S_Long-6

Plasmid

#197698

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S promoter (1.3kb)

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFTK019

Plasmid

#171291

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PTET-10x-repeat promoter+CPgpdA (fused)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK084

Plasmid

#171356

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

TtRNA[Met] A.niger Pol-III terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPD391 VPR-ZF37

Plasmid

#138839

Purpose

Constitutive expression of VPR-ZF37 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF37

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPD159 dsRedExpress2-ZFi43

Plasmid

#138799

Purpose

Constitutive expression of dsRedExpress2-ZFi43 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

dsRedExpress2-ZFi43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD539 VP16-ZF173

Plasmid

#138773

Purpose

Constitutive expression of VP16-ZF173 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF173

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD526 VP16-ZF13

Plasmid

#138755

Purpose

Constitutive expression of VP16-ZF13 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF13

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD528 VP16-ZF21

Plasmid

#138757

Purpose

Constitutive expression of VP16-ZF21 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF21

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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JPF0535a

Plasmid

#124042

Purpose

Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDIRECT_21D

Plasmid

#91131

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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