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pcDNA5-CEP41_Halo

Plasmid

#135446

Purpose

Mamalian cell expression of HaloTag7 localized at the centrosome

Depositor

Kai Johnsson

Publication

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CEP41-HaloTag7

Tags

CEP41

Expression

Mammalian

Promoter

CMV/TetO

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX6p1-HH1713

Plasmid

#124994

Purpose

E. coli expression of recombinant H. hepaticus antigen HH_1713

Depositor

Dan Littman

Publication

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH_1713

Expression

Bacterial

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMP11

Plasmid

#215559

Purpose

pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

Depositor

Brian Pfleger

Publication

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

Cas9
gRNA targeting pBR322 origin of replication

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23105 and tet

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNM3732

Plasmid

#153086

Purpose

mec-4p GFP-C1 positive control for RMCE integration

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p GFP-C1 tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pETO18G

Plasmid

#27401

Depositor

Jonathan Javitch

Publication

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Tags

HisX8 and Thrombin

Expression

Bacterial

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET3a-AqAdk

Plasmid

#18089

Depositor

Dorothee Kern

Publication

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad Aquifex aeolicus)

Expression

Bacterial

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac-CHD7

Plasmid

#170141

Purpose

Expresses CHD7 in insect cells

Depositor

Robert Kingston

Publication

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

6xHis and FLAG

Expression

Insect

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNM3823

Plasmid

#154123

Purpose

mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::tetR-L-QFAD::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3774

Plasmid

#154117

Purpose

tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆mec-7p::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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V143 pLP-HA SD - Acceptor

Plasmid

#11710

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

HA

Expression

Mammalian

Available since

June 22, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pPYRMandi-ABI

Plasmid

#233649

Purpose

Cellular colocalization assay (PYRMandi/ABI) for the CIP Mandi (mandipropamid)

Depositor

Richard Wombacher

Publication

Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TOMM20-mCherry-PYRMandi-P2A-eGFP-ABI

Expression

Mammalian

Promoter

CMV

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Publication

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h1

Plasmid

#86689

Purpose

For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Publication

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)

Tags

HA-tag

Promoter

T3

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CS-TRE-c-Myc-Ubc-rtTA

Plasmid

#198190

Purpose

Generation of Expandable megakaryocyte cell lines from human iPS cells

Depositor

Hiromitsu Nakauchi

Publication

Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

Insert

hC-MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA flag-Msi1

Plasmid

#184029

Purpose

Msi1 clone with N-terminal flag tag

Depositor

Peter Stoilov

Publication

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Msi1 (Msi1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Publication

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-MPHOSPH8 Chromo

Plasmid

#59694

Purpose

Contains histone modification interacting domain (MPHOSPH8 Chromo)

Depositor

Albert Jeltsch

Publication

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

MPHOSPH8 Chromo (MPHOSPH8 Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pQStrep2-ARPC3

Plasmid

#31580

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

Actin related protein 2/3 complex, subunit 3 (ARPC3 Human)

Tags

His and StrepII-tag

Expression

Bacterial

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSicoR-Ef1a-mCh-GFPi

Plasmid

#31849

Depositor

Bruce Conklin

Publication

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HS-H3.3A-GFP

Plasmid

#8696

Depositor

Kami Ahmad

Publication

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H3.3A (His3.3A Fly)

Tags

GFP

Expression

Insect

Available since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

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