Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 16,251 results for: She

Showing: 5201 - 5240 of 16251 results

  1. m.3075-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #211337
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-DddA11-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211337-plasmid-map-sequence-id-415646

    Enlarge

  2. pT7CFE1_EVA71_VP12A

    Plasmid
    #212977
    Purpose
    Encodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212977-plasmid-map-sequence-id-420362

    Enlarge

  3. pT7CFE1_EVD68_VP12A

    Plasmid
    #212984
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212984-plasmid-map-sequence-id-420357

    Enlarge

  4. pT7CFE1_EVD68_3ABC

    Plasmid
    #212979
    Purpose
    ncodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212979-plasmid-map-sequence-id-420356

    Enlarge

  5. pT7CFE1_EVD68_3ABCD

    Plasmid
    #212981
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212981-plasmid-map-sequence-id-420354

    Enlarge

  6. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208642-plasmid-map-sequence-id-418350

    Enlarge

  7. pENTR3cDual-Wnk

    Plasmid
    #203976
    Purpose
    Enrty clone for Gateway cloning
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203976-plasmid-map-sequence-id-403303

    Enlarge

  8. DV.7.1

    Plasmid
    #208584
    Purpose
    Mammalian expression vector for expressing DV.7.1 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant DV.7.1 spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208584-plasmid-map-sequence-id-409583

    Enlarge

  9. HA Display H3_A/Hong Kong/1/1968 Part 4-DO

    Plasmid
    #206518
    Purpose
    CcdB Dropout vector for part 4 of Hemagglutinin surface expression in mammalian cells
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    206518-plasmid-map-sequence-id-408109

    Enlarge

  10. HA Display H3_A/Hong Kong/1/1968 Part 1-DO

    Plasmid
    #206515
    Purpose
    CcdB Dropout vector for part 1 of Hemagglutinin surface expression in mammalian cells
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    206515-plasmid-map-sequence-id-408101

    Enlarge

  11. pAG416GAL-Bat-SL-CoVZC45_PLP

    Plasmid
    #201939
    Purpose
    Expresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    Bat-SL-CoVZC45 PLP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    201939-plasmid-map-sequence-id-402868

    Enlarge

  12. pAG416GAL10-Bat-SL-CoVZC45_3CL

    Plasmid
    #201944
    Purpose
    Expresses Bat-SL-CoVZC45 3CL protease from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    Bat-SL-CoVZC45 3CL protease
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    201944-plasmid-map-sequence-id-402788

    Enlarge

  13. pAG416GAL10-DUGV_OTU

    Plasmid
    #203463
    Purpose
    Expresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    DUGV OTU domain of L protein (DUGVsLgp1 )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203463-plasmid-map-sequence-id-402679

    Enlarge

  14. pAG416GAL-3x-uC-HCoV-229E_3CL

    Plasmid
    #201937
    Purpose
    Expresses HCoV-229E 3CL protease from a GAL-3x promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    HCoV-229E 3CL protease
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201937-plasmid-map-sequence-id-402673

    Enlarge

  15. pGMC00024 (aka pCW-Cas9n-Blast)

    Plasmid
    #195315
    Purpose
    Dox inducible lentiviral Cas9 nickase
    Depositor
    Raj Chari
    Insert
    Cas9-nickase
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A nickase
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    195315-plasmid-map-sequence-id-390525

    Enlarge

  16. pEXP1-DEST-Noa1-HIS

    Plasmid
    #191972
    Purpose
    Destination vector containing HIS-tagged NOA1 coding sequence.
    Depositor
    Luca Scorrano
    Insert
    Noa1-HIS (NOA1 Human)
    Use
    Synthetic Biology
    Tags
    His
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191972-plasmid-map-sequence-id-379639

    Enlarge

  17. pcDNA3-PARL-D319N-FLAG C-t

    Plasmid
    #191957
    Purpose
    Expression of Presenilin Associated Rhomboid Like (PARL) D319N mutant
    Depositor
    Luca Scorrano
    Insert
    PARL D319N (PARL Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D319N
    Promoter
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    191957-plasmid-map-sequence-id-379806

    Enlarge

  18. R6K_tac_dGFPmut3_ChInt

    Plasmid
    #187388
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Use
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187388-plasmid-map-sequence-id-370402

    Enlarge

  19. KBB96

    Plasmid
    #185113
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated so only one FXFG repeat remains
    Depositor
    Ken Belanger
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Last FXFG domain of Nup1
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185113-plasmid-map-sequence-id-368072

    Enlarge

  20. KBB109

    Plasmid
    #185066
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after NsiI restriction site
    Depositor
    Ken Belanger
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup1 C-terminal region, truncated at NsiI restric…
    Promoter
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    185066-plasmid-map-sequence-id-367178

    Enlarge

  21. pLZRS-TrkC-mCherry

    Plasmid
    #182949
    Purpose
    To express the rat TrkC fused in the C-terminus with mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    TRk-C-mCherry (Ntrk3 Synthetic, Rat)
    Use
    Retroviral and Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182949-plasmid-map-sequence-id-360115

    Enlarge

  22. pJA12 Mamu-A1*001:01

    Plasmid
    #180442
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-A1*001:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    180442-plasmid-map-sequence-id-355627

    Enlarge

  23. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid
    #177208
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    177208-plasmid-map-sequence-id-356946

    Enlarge

  24. pJA13 Mamu-A1*002:01

    Plasmid
    #180443
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-A1*002:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180443-plasmid-map-sequence-id-355622

    Enlarge

  25. pTCF122 Mamu-B*008:01

    Plasmid
    #180455
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-B*008:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180455-plasmid-map-sequence-id-355081

    Enlarge

  26. pTCF194 Mamu-E*02:04

    Plasmid
    #180461
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-E*02:04
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180461-plasmid-map-sequence-id-355080

    Enlarge

  27. SYKA-c014

    Plasmid
    #175488
    Purpose
    Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    SYK (SYK Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175488-plasmid-map-sequence-id-353252

    Enlarge

  28. pLV V5-Senp2

    Plasmid
    #175118
    Purpose
    Lentiviral expression of V5-tagged mouse Senp2
    Depositor
    Larry Gerace
    Insert
    Senp2 (Senp2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175118-plasmid-map-sequence-id-351807

    Enlarge

  29. RAB5AA-c006

    Plasmid
    #175476
    Purpose
    Protein expression in bacterial cells. GTPase domain, G15-N184. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    RAB5A (RAB5A Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175476-plasmid-map-sequence-id-353162

    Enlarge

  30. MSNA-c007

    Plasmid
    #175467
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-D298. Used for NMR studies.
    Depositor
    Opher Gileadi
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175467-plasmid-map-sequence-id-353136

    Enlarge

  31. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Renilla_shRNAmir

    Plasmid
    #178046
    Purpose
    CAG tdGFP Renilla shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Renilla_713 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178046-plasmid-map-sequence-id-352920

    Enlarge

  32. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir

    Plasmid
    #178045
    Purpose
    CAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Luciferase_1309 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178045-plasmid-map-sequence-id-352919

    Enlarge

  33. pSBbi-RB-PE2

    Plasmid
    #173902
    Purpose
    SB-transposon with constitutive expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173902-plasmid-map-sequence-id-346342

    Enlarge

  34. pKSB- attP 3xP3-GFPsv40 GGGG AAGA

    Plasmid
    #174534
    Purpose
    attP site and 3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    attP and 3xP3-GFP-SV40 BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    3xP3
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174534-plasmid-map-sequence-id-343228

    Enlarge

  35. pKSB- PUb-GFPsv40 GGGG AAGA

    Plasmid
    #174536
    Purpose
    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    PUb-GFP-SV40 BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174536-plasmid-map-sequence-id-343240

    Enlarge

  36. pKSB- PUb-GFPTub56D GGGG AAGA

    Plasmid
    #174537
    Purpose
    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes, with DmTub56D terminator
    Depositor
    Eric Marois
    Insert
    PUb-GFP-Tub56Dterminator BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174537-plasmid-map-sequence-id-343241

    Enlarge

  37. pISV-DsRed1-nopP

    Plasmid
    #171025
    Purpose
    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.
    Depositor
    Christian Staehelin
    Insert
    nopP
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2 X EN 35S
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    171025-plasmid-map-sequence-id-337715

    Enlarge

  38. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

    Enlarge

  39. PB CAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid
    #137857
    Purpose
    CAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP-GSAx9-iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    137857-plasmid-map-sequence-id-316181

    Enlarge

  40. rox Neo* rox

    Plasmid
    #134322
    Purpose
    rox-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    134322-plasmid-map-sequence-id-316179

    Enlarge

Showing: 5201 - 5240 of 16251 results