We narrowed to 9,406 results for: Coli
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Plasmid#53283PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pG108-K - AhpC-Glow
Plasmid#185135PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoterDepositorInsertsGlow gene
Bacteroides thetaiotaomicron ahpC promoter
UsePg106TagsExpressionBacterialMutationPromoterAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLz37_CB_operon_CsoS2_N1-N4
Plasmid#140856PurposeHalothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.DepositorInsertCbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
ins:CFP-NTR
Plasmid#66593PurposeSpecific and conditional cell ablation of beta cells in the zebrafish pancreasDepositorInsertInsulin promotor driving CFP-nitroreductase
UseZebrafish expressionTagsCFP fused with N-terminus of nitroreductaseExpressionMutationPromoterZebrafish Insulin promoter (approximately 1.2kb)Available SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
UbcH5B
Plasmid#60443PurposeExpress E2 UbcH5B in E. ColiDepositorAvailable SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAX
Plasmid#53274PurposeContains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationDeleted idi (ORF6) and crtX genesPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEC2861 (pSpy1C-PgyrA_ffluc)
Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisB-TRF2
Plasmid#50488PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 (TERF2 Human)
UseTags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-500PromoterpTrcAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Skp
Plasmid#213139PurposeExpresses chaperone protein SKPDepositorAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PAGFP_NS2B3
Plasmid#195755PurposeCloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.DepositorInsertDengue (Type-2) NS2B3
UseTagsPA-GFPExpressionMammalianMutationNo mutationsPromoterCMVAvailable SinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-3XNLS
Plasmid#209392PurposeTransiently expressing TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYS2cm_crtYf
Plasmid#85543PurposeConstitutive transcription of crRNA of crtYf, Chloramphenicol resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoterAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRT498-3xOtUBD
Plasmid#190088PurposeExpresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsert3xOtUBD
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR (in lacZ deficient strain)
Plasmid#99245PurposeFor autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable SinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET41c-PhTET2
Plasmid#182428PurposeExpresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coliDepositorInsertTetrahedral aminopeptidase TET2 from P. hirokoshii
UseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NR
Plasmid#89625PurposeExpresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG inductionDepositorInsertNitrate reductase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK1035
Plasmid#8832DepositorInsertTVMV protease
UseTagsHis and MBP (cleaved)ExpressionBacterialMutationsynthetic gene with codons optimized for E. coliPromoterAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
PARP2-c098
Plasmid#216873PurposeBacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-QF-T7-TetO-TetR_p15A
Plasmid#171661PurposeExpresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.DepositorInsertQF-T7-TetO-TetR
UseTagsExpressionBacterialMutationPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptac-sfGFP
Plasmid#139934PurposeIPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLacI-Ptac-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
UseTagsHisExpressionBacterialMutationPromoterAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
RNF146-c005
Plasmid#173091PurposeExpression of recombinant fusion protein in E. coliDepositorInsertRNF146 (RNF146 Human)
UseTagsmCeruleanExpressionBacterialMutationR99-L183, codon optimizedPromoterAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GDAP2-c005
Plasmid#173110PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015
Plasmid#202023PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
UseTagsExpressionBacterialMutationGvpA T6A-I48VPromoterAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPP076
Plasmid#158794PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-1
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM126
Plasmid#105134PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-GGPPipi
Plasmid#53280PurposeContains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coliDepositorInsertcrtE, idi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
UseTagsC terminal TEV GFP octa-His affinity tagExpressionBacterialMutationPromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE5400_pMBP_CDS dropout vector
Plasmid#221189PurposeExpression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interestDepositorTypeEmpty backboneUseTags10x His-tag + E. coli maltose binding protein (MB…ExpressionBacterialMutationPromoterT7 promoterAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHB-2
Plasmid#140955PurposeFor PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.DepositorInsertphaCAB
UseTagsExpressionBacterialMutationR238S mutation in PhaCPromoterAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
Plasmid#199763PurposeElongation reporter construct to quantify elongation of LacZDepositorInsertLacZ-nLucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETcLIC-GFP
Plasmid#183514PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coliDepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable SinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf (XSB522)
Plasmid#78512PurposeConstruct for expressing His10 - SNAPf in E. ColiDepositorInsertSNAPf
UseTagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutationPromoterAvailable SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pEF-ENTR-LacZ (710-1)
Plasmid#17430PurposeGateway entry vector with EF1-a promoter, V5-His C-term, Lac ZDepositorInsertLacZ
UseEntry vectorTagsHis and V5ExpressionMammalianMutationPromoterAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(+36GFP)-Cre
Plasmid#71748PurposeAurein1.2+36GFP-Cre fusion expression in E. ColiDepositorInsert(GGS)9_Aurein1.2+36GFP-Cre
UseTags6XHisExpressionBacterialMutationPromoterAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa
Plasmid#78182PurposeExpresses ffDronpa E. coli strainsDepositorInsertffDronpa
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCSGC-GB1
Plasmid#186786PurposeE. coli expression vector containing N-terminal 6His-GB1-TEV fusion.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gi-c001
Plasmid#173078PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptet-sfGFP
Plasmid#139935PurposeaTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertTetR-Ptet-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable SinceJuly 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
T. aq RNA Polymerase
Plasmid#215349PurposeExpress T. aq RNA Polymerase subunits in E. coli cellsDepositorInsertRNA polymerase
UseTags6x HisExpressionBacterialMutationPromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1
Plasmid#136647PurposeExpression of SHBP1-GST fusion protein in E. coliDepositorAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP2
Plasmid#26282DepositorInsertMSP2
UseTags6-HisExpressionBacterialMutationsynthetic gene of dimer of deletion mutant (1-43)…PromoterAvailable SinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
UseTags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-crRNA-Km
Plasmid#158712PurposePlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNADepositorInsertAcGFP (for crRNA cloning)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterTetOAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -