We narrowed to 4,720 results for: donor

Showing: 2601 - 2620 of 4720 results

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Aquamarine-LC3B

Plasmid

#228559

Purpose

Encodes a donor-only, Aquamarine-tagged version of the LC3B biosensor under the control of the CMV promoter

Depositor

Giulia Bertolin

Article

Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

Aquamarine

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1_dCas9-XTEN-KRAB-p2A-GFP

Plasmid

#222108

Purpose

Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)

Use

AAV and CRISPR

Tags

HA and P2A-GFP

Expression

Mammalian

Mutation

D10A, H840A

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB1-PGK-EGFP

Plasmid

#204018

Purpose

Homologous donor template for NFKB1 knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB1-PGK-tdTomato

Plasmid

#204019

Purpose

Homologous donor template for NFKB1 knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB2-PGK-EGFP

Plasmid

#204020

Purpose

Homologous donor template for NFKB2 knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB2-PGK-tdTomato

Plasmid

#204021

Purpose

Homologous donor template for NFKB2 knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBA791

Plasmid

#186246

Purpose

dnap-S HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBA792

Plasmid

#186247

Purpose

dnap-F HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-50-DExogron-mCherry-R11b

Plasmid

#179904

Purpose

Donor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMKRQ_11_DExCon-mNeonGreen-R25

Plasmid

#179896

Purpose

Donor with homologous arms for Rab25 to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB25, member RAS oncogene family (RAB25 Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-22-DExCon-mNeonGreen-R11a

Plasmid

#179897

Purpose

Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-23-DExCon-mCherry-R11b

Plasmid

#179898

Purpose

Donor with homologous arms for Rab11b to knock in DExCon-mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-67-DExCon-mNeonGreen-R11b

Plasmid

#179899

Purpose

Donor with homologous arms for Rab11b to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-59-DExCon-mTagBFP2-R25

Plasmid

#179900

Purpose

Donor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB25, member RAS oncogene family (RAB25 Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mTagBFP2

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

Plasmid

#168787

Purpose

Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology; Expression in fila…

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-E2

Plasmid

#175243

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Strep II

Plasmid

#153512

Purpose

Strep II C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Strep II

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TwinStrep

Plasmid

#153513

Purpose

TwinStrep C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TwinStrep

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Hsa-Serum Albumin SP

Plasmid

#153496

Purpose

Human Serum Albumin Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Serum Albumin SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Aaw-Glucoamylase SP

Plasmid

#153497

Purpose

Aspergillus awamori Glucoamylase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Glucoamylase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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