We narrowed to 3,800 results for: golden gate

1. ATP6 alphaDdCBE G1

   Plasmid

   #221958

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ATP6 alphaDdCBE T1

   Plasmid

   #221960

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ATP6 alphaDdCBE T2

   Plasmid

   #221961

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CO1 DdCBE C1

   Plasmid

   #221962

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-T-CO1 TALE C1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ATP6 DdCBE A1

   Plasmid

   #221946

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ATP6 DdCBE A2

   Plasmid

   #221947

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pro::EX1-2(intΔNAC2):GFP

   Plasmid

   #218574

   Purpose

   Transcriptional reporter for ARF7 promoter

   Depositor

   Anthony Bishopp

   Insert

   AT5G20730 (NPH4 Mustard Weed)
   

   Expression

   Plant

   Mutation

   Mutation genomic sequence 206nt, 207nt from CT to…

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pro::EX1-2(intΔNAC1):GFP

   Plasmid

   #218575

   Purpose

   Transcriptional reporter for ARF7 promoter

   Depositor

   Anthony Bishopp

   Insert

   AT5G20730 (NPH4 Mustard Weed)
   

   Expression

   Plant

   Mutation

   Mutation genomic sequence 85nt, 86nt from TA to CG

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pro::EX1-2(intΔNAC3):GFP

   Plasmid

   #218573

   Purpose

   Transcriptional reporter for ARF7 promoter

   Depositor

   Anthony Bishopp

   Insert

   AT5G20730 (NPH4 Mustard Weed)
   

   Expression

   Plant

   Mutation

   Mutation genomic sequence 334nt, 335nt from AG to…

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pro::EX1-2(intΔMYBs):GFP

    Plasmid

    #218571

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Expression

    Plant

    Mutation

    Mutation genomic sequence 187nt, 188nt from TG to…

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKL1-100

    Plasmid

    #219408

    Purpose

    Reporter gene (4 operators) for zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    (42-10 op)x4-minCyc1-Venus-tCyc1
    

    Expression

    Yeast

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKL1-422

    Plasmid

    #219410

    Purpose

    Reporter gene (1 operator) for zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    (42-10 op)x1-minCyc1-Venus-tCyc1
    

    Expression

    Yeast

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKL1-424

    Plasmid

    #219412

    Purpose

    Reporter gene (3 operator) for zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    (42-10 op)x3-minCyc1-Venus-tCyc1
    

    Expression

    Yeast

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKL1-095

    Plasmid

    #219407

    Purpose

    Reporter gene (4 operators) for zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    (13-6 op)x4-minCyc1-Venus-tCyc1
    

    Expression

    Yeast

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKL1-423

    Plasmid

    #219411

    Purpose

    Reporter gene (2 operator) for zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    (42-10 op)x2-minCyc1-Venus-tCyc1
    

    Expression

    Yeast

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L0-I26 pUBQ10 GG0-GG1

    Plasmid

    #195902

    Purpose

    Level 0 pUBQ10 promoter with GG0 and GG1 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pUBQ10 (UBQ10 Mustard Weed)
    

    Use

    Synthetic Biology

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOD_C'4800EC

    Plasmid

    #176875

    Purpose

    Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Cloning

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTSB-FICAT_Payload-Esp3I

    Plasmid

    #179380

    Purpose

    Transposon vector prepared for Esp3I golden gate cloning of Transgene

    Depositor

    Marc Güell

    Article

    Pallares-Masmitja et al Nat Commun. 2021 Dec 3;12(1):7071. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CSM160

    Plasmid

    #105684

    Purpose

    Plasmid containing an RFP dropout used to construct randomized PAM library

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    RFP Golden Gate dropout
    

    Expression

    Bacterial

    Promoter

    Bba_R0040 TetR Promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Sth_I-E array_GFP dropout

    Plasmid

    #178775

    Purpose

    Golden Gate GFP dropout vector to generate S. thermophilus type I-E single arrays

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    gfp dropout to generate S. thermophilus type I-E single arrays
    

    Expression

    Bacterial

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge