We narrowed to 2,753 results for: PGK
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Plasmid#127203PurposeConstitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchCDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
UseSynthetic BiologyAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5VY
Plasmid#165048PurposeIntegrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIEforRed
Plasmid#185892PurposeIn vivo gene amplification (HapAmp) of EforRed expression module at RPL25 locusDepositorInsertPRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
ExpressionYeastMutationNoneAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTKM31
Plasmid#233503PurposeLevel 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianusDepositorInsertPGK1-tetO2p
UseSynthetic BiologyExpressionYeastMutationaddition of two tetracycline operator sequenecesAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG144
Plasmid#227760PurposeExpresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationVal121Met mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG124
Plasmid#227758PurposeExpresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationThr83Ser mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_H2BK_mNG_hSLBP(18-108)
Plasmid#191529PurposeDonor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2BK_mNG_hSLBP(18-108)
UseCRISPR and Synthetic BiologyTagsmNeonGreen (mNG)ExpressionMammalianAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIAeBlueHPV16LR
Plasmid#185894PurposeIn vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locusDepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
ExpressionYeastMutationNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
cTRE-LSL-GFP-IRES-Cas9-sgCR8
Plasmid#135668PurposeIntroduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchangeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgCR8
UseMouse TargetingAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-CTBP2-MGMT_1
Plasmid#136419PurposeLentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-eGFP.PB/PTK
Plasmid#170540PurposeExpresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restoredDepositorInsertDelta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP
ExpressionMammalianAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-SA-CAG-Pa01-BFP
Plasmid#234142PurposePerturb Seq landing pad with Pa01 recombinase at the AAVS1 lociDepositorInsertPa01
ExpressionMammalianPromoterCAGAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTS2361_Tier3(SB)-rtTA-YPet_PuroR
Plasmid#169657PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2363_Tier3(PB)-rtTA-YPet_PuroR
Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305_CIC-DUX4
Plasmid#247361PurposeLentiviral constitutive expression construct for CIC::DUX4 (pLEX_305 backbone)DepositorUseLentiviralExpressionMammalianMutationFusion between CIC and DUX4PromoterhPGKAvailable sinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS403red-luxTA2-4F
Plasmid#166030PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJF87
Plasmid#253754PurposeEncodes GFP_AID* and pALD6-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.DepositorInsertsGFP_AID*
OsTIR1
TagsmAID*-tagExpressionYeastMutationF74G, S210APromoterALD6 and TEF1Available sinceApril 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-H4-HaloTag-IRES-Neo
Plasmid#247450PurposeExpresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposaseDepositorAvailable sinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEGF120TAG
Plasmid#200946PurposeEncodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporationDepositorInsertsUseLentiviralTags6x HISMutationamber stop codon 101 bp from ATGPromoterEf1a and hPGKAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only