We narrowed to 10,978 results for: Vars;

1. pDest17_A1-LCD swap X9

   Plasmid

   #226551

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD X9

   Depositor

   Tanja Mittag

   Article

   Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

   Insert

   A1-LCD swap X9
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDest17_A1-LCD swap X8

   Plasmid

   #226550

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD X8

   Depositor

   Tanja Mittag

   Article

   Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

   Insert

   A1-LCD swap X8
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDest17_A1-LCD swap X6

   Plasmid

   #226548

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD X6

   Depositor

   Tanja Mittag

   Article

   Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

   Insert

   A1-LCD swap X6
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDest17_A1-LCD swap X2

   Plasmid

   #226544

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD X2

   Depositor

   Tanja Mittag

   Article

   Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

   Insert

   A1-LCD swap X2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBJT031

   Plasmid

   #225177

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (AmpR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBJT034

   Plasmid

   #225180

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBJT032

   Plasmid

   #225178

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (TetR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHBJT033

   Plasmid

   #225179

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (GmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHBJT030

   Plasmid

   #225176

   Purpose

   Low copy cloning vector with mCherry integrated in the multiple cloning site (Chlr). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHBJT004

    Plasmid

    #225155

    Purpose

    Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHBJT003

    Plasmid

    #225154

    Purpose

    Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LAMTOR1-APEX2

    Plasmid

    #215556

    Purpose

    Stable, inducible expression in lysosome

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    LAMTOR1-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 5HT2A-APEX2

    Plasmid

    #215558

    Purpose

    Stable, inducible expression of 5HT2A

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAGK43

    Plasmid

    #222223

    Purpose

    Edits NPAS2 Gene.

    Depositor

    Seth Shipman

    Article

    Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSCL194

    Plasmid

    #222225

    Purpose

    Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    site 1/Cas9/Eco1 RT
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCL195

    Plasmid

    #222226

    Purpose

    Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    site 2/Cas9/Eco1 RT
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCL368

    Plasmid

    #222227

    Purpose

    Expresses site 3/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

    Insert

    site 3/Cas9/Eco1 RT
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_PA1472_Cterm_His

    Plasmid

    #220485

    Purpose

    heterologous expression of PA1472 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1472
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a_speG_Nterm_His

    Plasmid

    #220478

    Purpose

    heterologous expression for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    speG
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_PA4114_Nterm_His

    Plasmid

    #220479

    Purpose

    heterologous expression of PA4114 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA4114
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge