We narrowed to 26,261 results for: Vit

1. Circular-ic-CVB3-GFP

   Plasmid

   #226260

   Purpose

   Production of in situ circularizable RNA via IVT

   Depositor

   Prashant Mali

   Article

   Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

   Insert

   EGFP
   

   Use

   In vitro transcription

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Circular-ic-EMCV-GFP

   Plasmid

   #226259

   Purpose

   Production of in situ circularizable RNA via IVT

   Depositor

   Prashant Mali

   Article

   Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

   Insert

   EGFP
   

   Use

   In vitro transcription

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIG-VP16 RARa [TJ#389]

   Plasmid

   #16287

   Depositor

   Thomas Jessell

   Article

   Novitch et al Neuron. 2003 Sep 25. 40(1):81-95.

   Insert

   RAR alpha (RARA Human)
   

   Tags

   VP16

   Expression

   Mammalian

   Available Since

   Feb. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-HsSENP3

   Plasmid

   #71467

   Purpose

   Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA1-GATA6

   Plasmid

   #51929

   Purpose

   mammalian expression of mouse GATA6

   Depositor

   Jeffery Molkentin

   Article

   Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

   Insert

   GATA6 (Gata6 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-sapphireCKAR(T/A)

   Plasmid

   #118469

   Purpose

   Negative-control mutant for sapphireCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR(T/A)
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-CBE6a-V106W

   Plasmid

   #215824

   Purpose

   Express CBE6a-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 human A3C

   Plasmid

   #105047

   Purpose

   Overexpression of APOBEC3C in mammalian cell lines

   Depositor

   Carsten Münk

   Article

   Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

   Insert

   APOBEC3C (A3C) (APOBEC3C Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-blueAKAR(T/A)

   Plasmid

   #118471

   Purpose

   Negative-control mutant for blueAKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueAKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-mCherry::LANS4

    Plasmid

    #60785

    Purpose

    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

    Insert

    LANS4
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. hSTARR-seq_SCP1 vector

    Plasmid

    #99292

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-hL1

    Plasmid

    #89411

    Purpose

    mammalian expression of hL1

    Depositor

    Fritz Rathjen

    Article

    De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

    Insert

    L1CAM (L1CAM Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Akt-Scramble

    Plasmid

    #175014

    Purpose

    Negative-control construct for Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-Scramble
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Yellow Cameleon-Nano140/pcDNA3

    Plasmid

    #51966

    Purpose

    mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano140
    

    Expression

    Mammalian

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-RAB-EKARev(T/A)

    Plasmid

    #118475

    Purpose

    Negative-control mutant for RAB-EKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ExRai-AktAR(T/A)

    Plasmid

    #118463

    Purpose

    Negative-control mutant for ExRai-AktAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6T7

    Plasmid

    #71462

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-CRY2-mCh-superPLDx10

    Plasmid

    #188986

    Purpose

    A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Tags

    CRY2 and mCherry

    Expression

    Mammalian

    Mutation

    F163L, K249R, G328S, G381V, G429D, D480G

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-ABKAR

    Plasmid

    #140051

    Purpose

    Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. psiCHECK2-miR-34 WT

    Plasmid

    #78258

    Purpose

    psiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    fully complimentary miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Expression

    Mammalian

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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