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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,632 results for: t7

Showing: 2641 - 2660 of 10632 results

  1. pmCellFree_KA1_SARS-COV-2_S-frag5

    Plasmid
    #169404
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    C-term frag. S protein after the furin cleavag (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169404-plasmid-map-sequence-id-335424

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  11. phSOD1-L126X-N’His

    Plasmid
    #117707
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    L126X
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117707-plasmid-map-sequence-id-227541

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  12. phSOD1-D101G-N’His

    Plasmid
    #117704
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D101G
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117704-plasmid-map-sequence-id-227520

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  13. 5JJZ (CDYL2)

    Plasmid
    #101876
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDYL2 (CDYL2 Human)
    Tags
    6x His and AviTag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101876-plasmid-map-sequence-id-194525

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  14. pHL 2147

    Plasmid
    #69790
    Purpose
    KanR with FRT sites + pT7::LacO::SgrS::T1 terminator + ColE
    Depositor
    Han Lim
    Article
    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw
    Insert
    SgrS
    Use
    Synthetic Biology
    Tags
    LacO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pJK605

    Plasmid
    #72530
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    72530-plasmid-map-sequence-id-141993

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  16. pJK652

    Plasmid
    #72470
    Purpose
    Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)
    Depositor
    T. J. Kappock
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated histidine-233 to glycine
    Promoter
    T7
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72470-plasmid-map-sequence-id-134018

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  17. pJK623

    Plasmid
    #72540
    Purpose
    Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72540-plasmid-map-sequence-id-132632

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  18. pJK579

    Plasmid
    #72426
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites
    Depositor
    T. J. Kappock
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    4 silent mutations introduce unique internal SpeI…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72426-plasmid-map-sequence-id-133996

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  19. pJK624

    Plasmid
    #72539
    Purpose
    Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72539-plasmid-map-sequence-id-132631

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  20. pJK522

    Plasmid
    #72510
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72510-plasmid-map-sequence-id-132535

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Showing: 2641 - 2660 of 10632 results