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pBbS10a-RFP

Plasmid

#126173

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23116

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB18k-GFP

Plasmid

#126137

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB14c-GFP

Plasmid

#126129

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB13c-GFP

Plasmid

#126128

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS15a-RFP

Plasmid

#126190

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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EC.RNe.TYB1.a1-400.wt

Plasmid

#17949

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-400

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-400

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pNP154

Plasmid

#106360

Purpose

A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Type

Empty backbone

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago Chi-a

Plasmid

#14075

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-a

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-a

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-b

Plasmid

#14085

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-b

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-b

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-c

Plasmid

#14086

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-c

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-c

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
EC.RNe.TYB1.a1-395.wt

Plasmid

#17950

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-395

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-395

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
tdTurboRFP-pBAD

Plasmid

#54857

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 576

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and tdTurboRFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDESTFLAGHApuro3-Ago Chi-d

Plasmid

#14087

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-d

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-d

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pLV hUBC-stuffer-mCherry-P2A-PuroR

Plasmid

#253231

Purpose

Backbone for insert expression with mCherry-2A-PuroR from lentiviral vector with cloning sites

Depositor

Charles Gersbach

Article

Reisman et al bioRxiv 2025.10.11.681828

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pRS313 Gal1p2

Plasmid

#242948

Purpose

Backbone plasmid for IcsB and homologs subcloning

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

Backbone plasmid for subcloning

Tags

3X FLAG tag

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.1

Plasmid

#238542

Purpose

Golden gate acceptor backbone for position 1 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.2

Plasmid

#238543

Purpose

Golden gate acceptor backbone for position 2 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.3

Plasmid

#238544

Purpose

Golden gate acceptor backbone for position 3 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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