Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,118 results for: RIK

Showing: 4021 - 4050 of 6118 results

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pLenti-Zyxin-E (aa1-138)-EGFP

Plasmid

#187697

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa1-138

Promoter

CMV

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-G (aa1-372)-EGFP

Plasmid

#187699

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa1-372

Promoter

CMV

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-F (aa373-564)-EGFP

Plasmid

#187698

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa373-564

Promoter

CMV

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-C (aa139-371)-EGFP

Plasmid

#187695

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa139-371

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-A (aa139-564)-EGFP

Plasmid

#187693

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa139-564

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-B (aa309-564)-EGFP

Plasmid

#187694

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa309-564

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTE1069

Plasmid

#186629

Purpose

SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

Inserts

scbR
scbAp promoter

Use

Synthetic Biology

Tags

6xArg

Expression

Bacterial

Promoter

BBa_J23100 and scbAp

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_5-1

Plasmid

#185054

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_5_1_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_3-2

Plasmid

#185055

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_3_2_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_5-2

Plasmid

#185056

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_5_2_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

Plasmid

#158771

Purpose

Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-miRFP670-eDHFR(69K6)

Plasmid

#178852

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-mNG-eDHFR(69K6)

Plasmid

#178853

Purpose

Mammalian expression of mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

Plasmid

#105635

Purpose

Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid

Depositor

Thanos Halazonetis

Inserts

Rev7 (Mad2l2 Mouse)
Rev3l (REV3L Human)

Expression

Bacterial

Mutation

Mutations: F11S, G12A, V132K, C133V, A135K

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

Plasmid

#105637

Purpose

Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid

Depositor

Thanos Halazonetis

Inserts

Rev7 (Mad2l2 Mouse)
Rev3l (REV3L Human)

Expression

Bacterial

Mutation

Mutations: F11S, G12A, V132K, C133V, A135K

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-WT-eGFP-KDEL

Plasmid

#177727

Purpose

Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Tags

KDEL, Prolactin Signal Sequence, and eGFP

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p406 – TDH3-MTS-GFP11-Ura

Plasmid

#177733

Purpose

Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA marker

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

MTS-GFP11

Expression

Bacterial and Yeast

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-WT-KDEL

Plasmid

#177715

Purpose

Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Tags

KDEL and Prolactin Signal Sequence

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mSpata33-PA

Plasmid

#179129

Purpose

Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (Spata33 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin-TAPBPR 22-35

Plasmid

#153478

Purpose

Mammalian expression of FLAG-tagged Tapasin with TAPBPR a.a. 22-35

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

Tapasin a.a.10-20 replaced with TAPBPR a.a. 22-35

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin delta2step

Plasmid

#153479

Purpose

Mammalian expression of FLAG-tagged Tapasin delta2step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.13-14, 16-17

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin delta3step

Plasmid

#153480

Purpose

Mammalian expression of FLAG-tagged Tapasin delta3step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.12-14, 16-18

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin G15E

Plasmid

#153481

Purpose

Mammalian expression of FLAG-tagged Tapasin G15E

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

G15E

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin G15L

Plasmid

#153482

Purpose

Mammalian expression of FLAG-tagged Tapasin G15L

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

G15L

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin L18E

Plasmid

#153483

Purpose

Mammalian expression of FLAG-tagged Tapasin L18E

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

L18E

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-FLAG-Tapasin L18G

Plasmid

#153484

Purpose

Mammalian expression of FLAG-tagged Tapasin L18G

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

L18G

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-FLAG-Tapasin L18K

Plasmid

#153485

Purpose

Mammalian expression of FLAG-tagged Tapasin L18K

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

L18K

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-mSNX27-H112A RNAi-resistant

Plasmid

#163621

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

H112A and RNAi resistance

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAM1160c2

Plasmid

#159724

Purpose

Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MPGIL-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PREX2_DEP

Plasmid

#109893

Purpose

Protein expression and purification of PREX2_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PREX2_DEP (PREX2 Human)

Expression

Bacterial

Mutation

amino acids 369-474

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4021 - 4050 of 6118 results

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