We narrowed to 29,613 results for: des.2
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Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70
Plasmid#62953PurposeCreating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.DepositorInsert3XGal80-FLAG/HA/EGFP-Hsp70
Available SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]
Plasmid#66781PurposeGly426 of miniSOG was mutated to 426Cys in tomm-20N::miniSOGDepositorInsertminiSOG
Tagstomm-20 (N terminal 55 aa)ExpressionWormMutationG426CAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
Plasmid#159874PurposeThree-fragment Gateway compatible protein tag for expression in C. elegansDepositorInsert[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70
Plasmid#78307PurposeCreating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-nlsLexA::GADflluw
Available SinceJuly 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Methyl-binding domain protein 2 (MBD2) coiled-coil domain
Plasmid#132721PurposeBacterial expression vector for methyl-binding domain protein 2 (MBD2) coiled-coil domain for use as competitor peptide in measurements of effective concentrationsDepositorInsertMethyl-binding protein domain 2 coiled-coil domain
TagsN-terminal His-tag. Flanking linkers to match acc…ExpressionBacterialPromoterT/Available SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)
Plasmid#178997PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)
Plasmid#154824Purpose(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegansDepositorInsertPeft-3::wrmScarlet::tbb-2 UTR
ExpressionWormPromotereef-1A.1 (eft-3)Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI
Plasmid#192112PurposeExpresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-BA.4/5 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2
Plasmid#182359PurposePositive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.DepositorInsertUltra-pan-neuronal positive control pUNP::gfp(NLS)
ExpressionWormPromoterPromoter Ultra Pan NeuronalAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75233PurposeCRISPR/Cas9 plasmid against human NFATc2DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
Plasmid#101232PurposeBacterial expression plasmid for SpCas9 Cluster 2 + Q926A variantDepositorInsertSpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationG528A, V583A, E584A, D585A, N588A and Q926APromoterT7Available SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry
Plasmid#208835PurposeKnockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoterDepositorInsertmCherry
UseAAV and CRISPRExpressionBacterial and MammalianPromoterhSyn1Available SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry
Plasmid#208836PurposeKnockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoterDepositorInsertmCherry
UseAAV and CRISPRExpressionBacterial and MammalianPromoterhSyn1Available SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75237PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-WT
Plasmid#218455PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying an N-terminal GST-tag (S SARS-CoV-2)
TagsGSTExpressionBacterialAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75241PurposeCRISPR/Cas9 plasmid against human IkarosDepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050
Plasmid#180644PurposeMammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.DepositorInsertULK1 (ULK1 Human)
TagsOne-Strep-Flag (OSF)ExpressionMammalianMutationResidues 833-1050PromoterpCAGAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2
Plasmid#24419DepositorInsertRS1 (HTR4 Human)
UseMouse TargetingTagsFLAG and mCherryMutationHuman 5HT4B receptor with D100A mutation, generat…Available SinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Glu
Plasmid#218458PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Glu substitution) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying Thr1273Glu substitution and an N-terminal GST-tag (S SARS-CoV-2)
TagsGSTExpressionBacterialMutationThr1273Glu substitutionAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only