We narrowed to 4,947 results for: mcs

1. OPEN-HLA-B*07:02-BirA

   Plasmid

   #239752

   Purpose

   E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-B*07:02 (HLA-B Human)
   

   Tags

   BirA substrate peptide

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   T7

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-RAB10 T23N

   Plasmid

   #130885

   Purpose

   Expresses human RAB10 T23N mutant with N-terminal GFP tag

   Depositor

   Michael Bassik

   Article

   Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

   Insert

   RAB10 (RAB10 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T23N

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. yeast_DualReporter

   Plasmid

   #127546

   Purpose

   A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).

   Depositor

   Aviv Regev

   Article

   de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do

   Inserts

   bla
   mCherry
   URA3 (URA3 Budding Yeast)
   yEVenus
   Nat1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Rab27B Q78L

   Plasmid

   #89448

   Purpose

   Expression of GFP-tagged human Rab27B Q78L in mammalian cells

   Depositor

   Wendy Westbroek

   Article

   Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

   Insert

   human Rab27B Q78L (RAB27B Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Q78L

   Promoter

   CMV

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV 4xSTAR-mTagBFP2-blast

   Plasmid

   #125239

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mTagBFP2 (codon optimized)

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIRCNpu

   Plasmid

   #74226

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Npu DnaE C-intein and Npu DnaE N-intein

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Myc-AIM2

   Plasmid

   #73958

   Purpose

   Express c-Myc-tagged human AIM2 in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

   Insert

   absent in melanoma 2 (AIM2) (AIM2 Human)
   

   Tags

   c-myc

   Expression

   Mammalian

   Promoter

   CMV iE

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRetroX-Tight_A-CREB_PGK-tdTomato

   Plasmid

   #154264

   Purpose

   Dox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   dominant negative CREB
   

   Expression

   Mammalian

   Promoter

   Tight/tetO

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-Crimson

   Plasmid

   #70683

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

   Depositor

   Daniel Bauer

   Article

   Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

   Inserts

   S. pyogenes sgRNA cassette
   Crimson Reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-a and hU6

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-Rab27B N133I

    Plasmid

    #89449

    Purpose

    Expression of GFP-tagged human Rab27B N133I in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B N133I (RAB27B Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    N133I

    Promoter

    CMV

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Rab27B T23N

    Plasmid

    #89450

    Purpose

    Expression of GFP-tagged human Rab27B T23N in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B T23N (RAB27B Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    T23N

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIZ-EGFP-MYO10-HMM

    Plasmid

    #87257

    Purpose

    Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Expression

    Insect

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    OpIE2

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-HA-GOLPH3

    Plasmid

    #21687

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-SARS-CoV Δ18

    Plasmid

    #169465

    Purpose

    Encodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. VRK1-dTag-puro

    Plasmid

    #199651

    Purpose

    Expresses VRK1 C-term. Tagged with dTAG

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    dTAG

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-Rab8a-WT

    Plasmid

    #101048

    Purpose

    Expresses HA-tagged human Rab8-WT in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-Rab8a-CA (Q67L)

    Plasmid

    #101050

    Purpose

    Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AICSDP-34: TNNI1-mEGFP

    Plasmid

    #114411

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEvol-pAcFRS.1.t1

    Plasmid

    #73545

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.1.t1
    pAcFRS.1.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX317-VRK1-K179E

    Plasmid

    #199644

    Purpose

    Expresses kinase-inactive VRK1 (K179E)

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Mutation

    K179E

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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