We narrowed to 5,111 results for: codon optimized

1. pscALPS- HIV2-ROD vpx

   Plasmid

   #115816

   Purpose

   Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   HIV2ROD Vpx (M15390)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Promoter

   SFFV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SQT1659

   Plasmid

   #78743

   Purpose

   Human expression plasmid for AsCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_dCas9-2xAM

   Plasmid

   #92220

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA to be inserted into Bbs I sites
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-parkin WT

   Plasmid

   #45969

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (Prkn Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression

   Promoter

   tac

   Available Since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. WN10151

   Plasmid

   #80441

   Purpose

   Expresses human codon-optimized dead (832A) LbCpf1

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Insert

   dead (D832A) LbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   D832A

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD315 (mTurquoise2^SEC^2xHA)

   Plasmid

   #73343

   Purpose

   mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

   Insert

   mTurquoise2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   2xHA and C. elegans codon-optimized mTurquoise2

   Expression

   Worm

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP hGMCSF co

   Plasmid

   #74168

   Purpose

   Lentiviral vector for expression of human codon optimized GMCSF with puromycin resistance.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GMCSF (CSF2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK2139

   Plasmid

   #65776

   Purpose

   Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-parkin

   Plasmid

   #227367

   Purpose

   For bacterial expression of parkin

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin (PRKN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression.

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HCMVgH (TB40/E)

    Plasmid

    #136446

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HDM_RSV_B1_F

    Plasmid

    #237353

    Purpose

    Expression plasmid for RSV B1 F

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV B1 F human codon optimized from Genbank Accesion AF013254
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-memRFP-3xPax7gRNA

    Plasmid

    #224569

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC6A3

    Plasmid

    #132160

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Sijben et al Sci Rep. 2021 Jan 14;11(1):1312. doi: 10

    Insert

    SLC6A3 (SLC6A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pHUC-JEDI-1P

    Plasmid

    #202619

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P under the HuC promoter for pan neuronal expression in zebrafish; JEDI-1P sequence codon-optimized for zebrafish expression

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    Zebrafish expression

    Promoter

    HuC(elavl3)

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NylCA_pET28a

    Plasmid

    #215418

    Purpose

    Expression construct for NylCA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCA
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. NylCp2_pET28a

    Plasmid

    #215420

    Purpose

    Expression construct for NylCp2 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCp2
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDNR-SpCas9

    Plasmid

    #80425

    Purpose

    Used for in vitro transcription of SpCas9 mRNA.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    none

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid

    #47550

    Purpose

    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    ttTi5605 sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. APLF-c001

    Plasmid

    #173077

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    APLF (APLF Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K360-V448, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKMD106e - intein-Cas9(S219)

    Plasmid

    #64190

    Purpose

    Expresses intein-Cas9(S219) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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