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pJMC-3T

Plasmid

#197789

Purpose

Level 1 Module 3T destination vector

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS271

Plasmid

#73722

Purpose

SapTrap SNAPtag + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Snaptag + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFH80

Plasmid

#128191

Purpose

Level1 ZmUBIp::xCas9 3.7:NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::xCas9 3.7:NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTF-R

Plasmid

#139669

Purpose

Expression cassette for R recombinase in T-DNA vector

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

R recombinase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pPTK017-3b-yEGFP_Sec

Plasmid

#84976

Purpose

Type 3b, Green fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFH76

Plasmid

#125815

Purpose

Level0 ScCas9, wheat codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ScCas9, wheat codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS254

Plasmid

#73721

Purpose

SapTrap Halotag + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Halotag + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS297

Plasmid

#73737

Purpose

SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

STOP codon + let-858 3'-UTR

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pPTK015-3-yEGFP_Intra

Plasmid

#84974

Purpose

Type 3, Green fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK019-4-tAOX1

Plasmid

#84988

Purpose

Type 4, tAOX, Alcohol oxidase 1 terminator

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 terminator

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFLPe-AntiDsRed

Plasmid

#139676

Purpose

Cassette for testing FLPe recombinase activity using a fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed FLPe

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBALU-ext

Plasmid

#69339

Purpose

It allows to extend the genomic insert in a fosmid.

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

kanamycin gene +pCC1FOS homology

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pMLS269

Plasmid

#73727

Purpose

SapTrap SL2::SV40-NLS C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-nlsGFP pA

Plasmid

#80814

Purpose

nlsGFP with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

nlsGFPpA 3'E

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFH38

Plasmid

#126012

Purpose

Level0 Rice OsU3 Pol III promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsU3 Pol III promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH16

Plasmid

#125801

Purpose

Level0 FnCas12a, rice codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

FnCas12a, rice codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_lacI_Plac

Plasmid

#243920

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI and PlacZYA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_tetR_Ptet

Plasmid

#243924

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_cI857_PR

Plasmid

#243926

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7

Plasmid

#243928

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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