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pRegev_S2

Plasmid

#204162

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S2 exon in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D4.34C>G

Plasmid

#204185

Purpose

pRegev_D4 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D4 with 34C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

34C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D3.48C>G

Plasmid

#204183

Purpose

pRegev_D3 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3 with 48C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

48C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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SL-TREAT

Plasmid

#196918

Purpose

The TREAT reporter containing cap-proximal GC-rich stemloop

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(G41)

Plasmid

#197939

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(G41S)

Plasmid

#197940

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(V97T)

Plasmid

#197941

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_Halo-PanK

Plasmid

#197948

Purpose

Expression of FRET_control in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pProbe_CUSPER

Plasmid

#185953

Purpose

broad host plasmid that can be used to construct reproductive success bioreporter

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al ISME J. 2023 Sep;17(9):1445-1454. doi: 1

Insert

Unspecified

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSMNot_sra-6::trp-4

Plasmid

#182261

Purpose

trp-4 cDNA cloned into pSMNot_sra-6 at Kpn&Notl

Depositor

Sreekanth Chalasani

Article

Ibsen et al Nat Commun. 2015 Sep 15;6:8264. doi: 10.

Inserts

trp-4 (trp-4 Nematode)
sra-6 (sra-6 Nematode)

Expression

Bacterial

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSecEffector wo TEVp

Plasmid

#172892

Purpose

atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

Bxb1
TP901

Expression

Bacterial

Promoter

nTETO and pBAD /AraC

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSMNot_odr-3::trp-4

Plasmid

#182262

Purpose

trp-4 cDNA cloned into pSMNot_odr-3 at KpnI & NotI

Depositor

Sreekanth Chalasani

Article

Ibsen et al Nat Commun. 2015 Sep 15;6:8264. doi: 10.

Inserts

trp-4 (trp-4 Nematode)
odr-3 (odr-3 Nematode)

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0W0VV04

Plasmid

#163239

Purpose

Inducible expression of UniProt identifier A0A0W0VV04

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0W0VV04

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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D4XIR1

Plasmid

#163200

Purpose

Inducible expression of UniProt identifier D4XIR1

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

D4XIR1

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pZA70

Plasmid

#174784

Purpose

RNAi

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

Intron-containing hairpin RNA (ihpRNA)-NbJIM2

Use

RNAi

Mutation

WT

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huMlCas12a

Plasmid

#174678

Purpose

Expresses 6xHis-MBP tagged MlCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huMlCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huBfCas12a

Plasmid

#174672

Purpose

Expresses 6xHis-MBP tagged BfCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huBfCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKAn-yibDp-Q-GRFT

Plasmid

#158748

Purpose

low phosphate yibDp induction of Q-Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

yibDp Q-Griffithsin

Expression

Bacterial

Mutation

M78Q

Promoter

yibD

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKAn-phoBp-GRFT

Plasmid

#158746

Purpose

low phosphate phoBp induction of Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

phoBp Griffithsin

Expression

Bacterial

Promoter

phoB

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-eCas9-D1135E

Plasmid

#154926

Purpose

aTc inducible eCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

eCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

K848A, K1003A, R1060A, D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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