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We narrowed to 44,705 results for: cha

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Showing: 2741 - 2760 of 44705 results

  1. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  3. pAAV-CAG-DIO-ChR2(H134R)-eYFP

    Plasmid
    #127090
    Purpose
    Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation
    Depositor
    Viviana Gradinaru
    Article
    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin-2
    Use
    AAV
    Tags
    eYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    Humanized ChR2 gene with histidine 134 changed to…
    Promoter
    CAG
    Available Since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127090-plasmid-map-sequence-id-246739

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  4. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

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  6. pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)

    Plasmid
    #55614
    Purpose
    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells
    Depositor
    David Chan
    Article
    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.
    Insert
    3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
    Tags
    3xFLAG and STREP
    Expression
    Mammalian
    Mutation
    C4762 mutated to serine to render HERC2 catalytic…
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    55614-plasmid-map-sequence-id-86500

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  9. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey

    Plasmid
    #238132
    Purpose
    This plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238132-plasmid-map-sequence-id-476568

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  10. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster

    Plasmid
    #238131
    Purpose
    This plasmid encodes the full-length hamster (Mesocricetus auratus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238131-plasmid-map-sequence-id-476564

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  11. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid
    #201955
    Purpose
    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201955-plasmid-map-sequence-id-405309

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  12. RPL25-GFP (94)

    Plasmid
    #24037
    Depositor
    Michael Rout
    Insert
    RPL25 (RPL25 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    March 10, 2010
    Availability
    Academic Institutions and Nonprofits only

  13. RPS1B-GFP (95)

    Plasmid
    #24039
    Depositor
    Michael Rout
    Insert
    RPS1B (RPS1B Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    March 31, 2010
    Availability
    Academic Institutions and Nonprofits only

  14. RPL3-GFP (96)

    Plasmid
    #24040
    Depositor
    Michael Rout
    Insert
    RPL3 (RPL3 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    March 31, 2010
    Availability
    Academic Institutions and Nonprofits only

  15. RPL25NLS-GFP (101)

    Plasmid
    #24038
    Depositor
    Michael Rout
    Insert
    RPL25 (RPL25 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Only bp 1-135 (aa 1-45) of RPL25
    Available Since
    March 10, 2010
    Availability
    Academic Institutions and Nonprofits only

  16. PHO4NLS-GFP-PRA (109)

    Plasmid
    #24044
    Depositor
    Michael Rout
    Insert
    PHO4
    Tags
    EGFP and PrA
    Expression
    Yeast
    Mutation
    Only bp 421-588 of PHO4 (NLS)
    Available Since
    May 3, 2010
    Availability
    Academic Institutions and Nonprofits only

  17. NAB2NLS-GFP-PRA (107)

    Plasmid
    #24043
    Depositor
    Michael Rout
    Insert
    NAB2
    Tags
    EGFP and PrA
    Expression
    Yeast
    Mutation
    Only bp 601-750 of NAB2 (NLS)
    Available Since
    May 3, 2010
    Availability
    Academic Institutions and Nonprofits only

  18. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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  19. RPL25NLS-GFP-PRA (102)

    Plasmid
    #24041
    Depositor
    Michael Rout
    Insert
    RPL25 (RPL25 Budding Yeast)
    Tags
    EGFP and PRA
    Expression
    Yeast
    Mutation
    Only bp 1-135 (aa 1-45) of RPL25
    Available Since
    May 3, 2010
    Availability
    Academic Institutions and Nonprofits only

  20. KAP95-FLU (113)

    Plasmid
    #24045
    Depositor
    Michael Rout
    Insert
    KAP95 (KAP95 Budding Yeast)
    Tags
    FLU
    Expression
    Yeast
    Available Since
    March 29, 2010
    Availability
    Academic Institutions and Nonprofits only
Showing: 2741 - 2760 of 44705 results