We narrowed to 5,111 results for: codon optimized

1. AfNitB_pET29b

   Plasmid

   #215401

   Purpose

   Expression construct for AfNitB gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   AfNitB
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-intron-BaLgp160

   Plasmid

   #100918

   Purpose

   Mammalian expression plasmid for Env from the BaL HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BaL) Env with upstream intron
   

   Tags

   CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL2783 (pTnsC-VP64_Vch)

   Plasmid

   #200896

   Purpose

   Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchTnsC-VP64
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3-SaCas9-BlastR

   Plasmid

   #220966

   Purpose

   Expresses human codon-optimized SaCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SaCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-mCherry-C1-GtACR1

   Plasmid

   #85464

   Purpose

   Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

   Depositor

   Peter Hegemann

   Article

   Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

   Insert

   Synthetic construct ACR1 gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV (+enhancer)

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-Blast coUGP2

   Plasmid

   #211531

   Purpose

   Expresses codon optimized UGP2

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   UGP2 (UGP2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. NylB'_pET21b

   Plasmid

   #215416

   Purpose

   Expression construct for NylB' gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB'
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-mCherry-C1-GtACR2

   Plasmid

   #85463

   Purpose

   Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

   Depositor

   Peter Hegemann

   Article

   Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

   Insert

   Synthetic construct ACR2 gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV (+enhancer)

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

   Plasmid

   #70658

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-T3-CjCAS9-pA

    Plasmid

    #131543

    Purpose

    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    FLAG-SV40NLS, SV40NLS, and polyA

    Expression

    Mammalian

    Mutation

    Human codon-optimized

    Promoter

    CAG, T3

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVRa41_491

    Plasmid

    #49702

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF41_491
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTU422

    Plasmid

    #178982

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Inserts

    gRAMP
    CRISPR array
    

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSmart-EF1a-Nla-cas5

    Plasmid

    #178878

    Purpose

    Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas5c-bGH polyA
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSmart-EF1a-Nla-cas8

    Plasmid

    #178880

    Purpose

    Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas8c-bGH PolyA
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSmart-EF1a-Nla-cas11

    Plasmid

    #178882

    Purpose

    Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas11c-bGH polyA
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSmart-EF1a-Nla-cas7

    Plasmid

    #178879

    Purpose

    Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas7c-bGH PolyA
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. ARH1-c003

    Plasmid

    #173093

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRH (ADPRH Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E2-L357, codon optimized

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL2669 (pTnsABf_Vch)

    Plasmid

    #200897

    Purpose

    Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsA-TnsB
    

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ALC1-c005

    Plasmid

    #173092

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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