We narrowed to 3,483 results for: mpo

1. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

   Plasmid

   #216479

   Purpose

   lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   wtYAP (YAP1) (YAP1 Human)
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

   Plasmid

   #159697

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEVI(408)-ColE-iLight-msfGFP

   Plasmid

   #170268

   Purpose

   Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   iLight
   

   Tags

   msfGFP

   Expression

   Bacterial

   Mutation

   To design iLight for expression in bacteria, a fu…

   Promoter

   pJ23116

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-EF-FH-TAZ S89A-ires-blast

   Plasmid

   #52084

   Purpose

   Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

   Depositor

   Yutaka Hata

   Article

   Yang et al Mol Cell Biol. 2014 Feb 18.

   Insert

   TAZ S89A (WWTR1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and His

   Expression

   Mammalian

   Mutation

   S89A

   Promoter

   EF1a

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EF-FH-TAZ-ires-blast

   Plasmid

   #52083

   Purpose

   Lentiviral expression vector for TAZ with N-terminal FLAG and His tags

   Depositor

   Yutaka Hata

   Article

   Yang et al Mol Cell Biol. 2014 Feb 18.

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and His

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-Tet-O-RUNX1T1-Puro

   Plasmid

   #221666

   Purpose

   Expresses RUNX1T1 in mammalian cells

   Depositor

   Vanessa Hall

   Article

   Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

   Insert

   RUNX1T1 (RUNX1T1 Human)
   

   Use

   Lentiviral

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

   Plasmid

   #159696

   Purpose

   E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
   

   Tags

   His6-Halotag-tev

   Expression

   Bacterial

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

   Plasmid

   #52504

   Purpose

   mammalian expression vector of BRCA1 wildtype siRNA resistant

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: AGTATAATC

   Promoter

   CMV

   Available Since

   Feb. 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pQCXIZ GFP PODXL delta PBM + Ezrin

   Plasmid

   #202418

   Purpose

   Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   Ezrin and GFP

   Mutation

   delete PDZ binding motif

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP

    Plasmid

    #216480

    Purpose

    lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    TAZ4SA (WWTR1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Mutation

    S66A, S89A, S117A, S311A

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP

    Plasmid

    #210513

    Purpose

    expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Inserts

    secreted alkaline phosphatase
    CMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV and TetO

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

    Plasmid

    #159698

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CMV TRE3G Neo GFP-Progerin C661S

    Plasmid

    #118711

    Purpose

    Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

    Depositor

    Tom Misteli

    Article

    Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

    Insert

    GFP-Progerin C661S (LMNA Human)
    

    Use

    Lentiviral; Tet-on inducible

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C661S farnesylation mutant

    Promoter

    CMV TRE3G (TET-ON)

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-NanoLuc-BRAF-CAT

    Plasmid

    #194772

    Purpose

    Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.

    Depositor

    Deborah Morrison

    Article

    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

    Insert

    BRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
    

    Tags

    NanoLuc

    Expression

    Mammalian

    Promoter

    CMV51

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-BRAF-REG-Halo

    Plasmid

    #194771

    Purpose

    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

    Depositor

    Deborah Morrison

    Article

    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

    Insert

    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV51

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. R725-X11-566: His6-MBP-tev-Hs.LGALS3

    Plasmid

    #159699

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.LGALS3

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

    Plasmid

    #169160

    Purpose

    To express nsp14-nsp10 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-CMV-EGFP-PolB-PAMmut-Hygro

    Plasmid

    #176086

    Purpose

    EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by PolBKOg1

    Promoter

    CMV

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

    Plasmid

    #159700

    Purpose

    E. coli protein expression

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)
    

    Tags

    His6-MBP-tev-Avi

    Expression

    Bacterial

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

    Plasmid

    #169189

    Purpose

    Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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