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pcDNA4-GFP-Metavinculin
Plasmid#107151Purposemammalian expressionDepositorInsertmetavinculin (VCL Human)
UseTags(His)10-GFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sender plasmid
Plasmid#127677PurposeEncodes the synthase LuxI for the quorum-sensing molecule C6-HSL.DepositorInsertLuxI
UseSynthetic BiologyTagsLVAExpressionBacterialMutationPromoterpT7Available sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pGEX3x-hRanBP2-(IR1+M)
Plasmid#53140PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsGSTExpressionBacterialMutationfragment (amino acids 2631-2711)PromotertacAvailable sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
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KA2628_pGL4-UBCmin-luc2/Tbx3-B'
Plasmid#124201PurposeLuciferase reporter driven by the Tbx3 enhancerDepositorInsertTbx3 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)-mSandy2
Plasmid#177760PurposeExpresses mSandy2 using the T7 PromoterDepositorInsertmSandy2
UseTags6xHis tagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDM4-GLY
Plasmid#49808PurposeEncodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.DepositorInsertsphosphoglycerate kinase
glyceraldehyde-3-phosphate dehydrogenase
pyruvate dehydrogenase subunit E1
dihydrolipoamide acetyltransferase
dihydrolipoamide dehydrogenase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046795
Plasmid#145598PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
RanBP2-RB3-4-pET23a
Plasmid#85769Purposebacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tagDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsHisExpressionBacterialMutationfragment (amino acids 2304-3062)PromoterT7Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Mycn-IRES-RFP
Plasmid#35393DepositorInsertsUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046740
Plasmid#145588PurposeBacterial Expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-PLS-KP6_rpoA-U
Plasmid#190967PurposeTest the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHL 1903
Plasmid#53020PurposeAmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsAAV, Asp terminator, and fimSExpressionBacterialMutationPromoterfimSAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMAH-EcaYRS
Plasmid#153558PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in mammalian cellsDepositorInsertsEcaYRS
amber suppression tRNA (multiple copies)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3-Neo
Plasmid#69123PurposeSleeping Beauty (SB) transposon with G418 resistanceDepositorInsertNeo (codon optimized for human)
UseSleeping beauty transposonTagsExpressionMammalianMutationPromoterU3MSCVAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2851
Plasmid#87761PurposePunc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.DepositorInsertPunc-17b-PH-miniSOG(Q103L)
UseTagsPHExpressionWormMutationPromoterPunc-17bAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046747
Plasmid#149249PurposeBacterial expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046733
Plasmid#145665PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CIBN-CreC
Plasmid#75267PurposeThe first half of the photo-activatable cre recombinaseDepositorInsertN-terminal portion of the CIB1 transcription factor
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
SP6-hCas9-Ce-mRNA
Plasmid#47911PurposeIn vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.DepositorInserthCas9 with C. elegans 5' and 3' UTRs
UseCRISPRTagsExpressionMutationPromoterSP6Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046916
Plasmid#145627PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-HA-NLGN4 D396*
Plasmid#59321PurposeExpression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutationDepositorInsertNLGN4 D396* (NLGN4X Human)
UseTagsHAExpressionMammalianMutationD396* nonsense mutation, rest of ORF deletedPromoterCAGAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
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POM42-mCherry
Plasmid#69856PurposeN-terminal tagging with mCherry using URA3 as a selection marker in S. cerevisiae.DepositorInsertmCherry minus start and stop codon
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/plsA
Plasmid#73625PurposeProduces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tagDepositorInsertadenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046731
Plasmid#145633PurposeBacterial Expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046871
Plasmid#145724PurposeBacterial Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
KA1158_pPBCAG-MerCreMer-IZ
Plasmid#124184PurposepiggyBac-based expression of the tamoxifen-inducible CreDepositorInsertMerCreMer-IRES-Zeo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only
