We narrowed to 4,010 results for: biorxiv

1. Efg1-ΔDBD-Y478S/Y482S/Y484S

   Plasmid

   #216411

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Y478S/Y482S/Y484S
   

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Tyrosine 478, 482 and 484 to Serine, and …

   Available Since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-MBP-TEVpCS-ADGRA1-FLAG

   Plasmid

   #217678

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRA1 (ADGRA1 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210736

   Purpose

   Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Merry sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210735

   Purpose

   Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Gollum sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-GST-ATG13 (363-398)-F394D/M396D/F397D

   Plasmid

   #210848

   Purpose

   Mammalian expression of ATG13 (363-398) mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Expression

   Mammalian

   Mutation

   F394D/M396D/F397D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-GST-ATG13 (363-450)-F394D/F397D /E403K

   Plasmid

   #210850

   Purpose

   Mammalian expression of ATG13 (363-450) mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Expression

   Mammalian

   Mutation

   F394D/F397D /E403K

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR4 CDS A. thaliana MAMI

   Plasmid

   #209186

   Purpose

   pENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for N-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   AT5G54110.1 (MAMI Mustard Weed)
   

   Use

   Gateway-compatible entry vector

   Promoter

   none

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR4 genomic A. thaliana PLDGAMMA3

   Plasmid

   #209188

   Purpose

   pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   AT4G11840 (PLDGAMMA3 Mustard Weed)
   

   Use

   Gateway-compatible entry vector

   Promoter

   none

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mc2 blast hPGK

   Plasmid

   #206229

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mc2 hygro hPGK

    Plasmid

    #206228

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mc2 puro hPGK

    Plasmid

    #206227

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mc2 neo hPGK

    Plasmid

    #206226

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mc2 hygro EF1a

    Plasmid

    #206222

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mc2 hygro

    Plasmid

    #206216

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202024

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
    

    Expression

    Bacterial

    Mutation

    GvpA T6A-L40A

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TEG +Q111T

    Plasmid

    #187435

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed glutamine at 117 to threonine

    Promoter

    PH and p10

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pminiT-MED6-mEmerald donor

    Plasmid

    #197865

    Purpose

    Donor plasmid used for building MED6-mEmerald knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    Med6 (Med6 Mouse)
    

    Use

    Mouse Targeting

    Tags

    mEmerald

    Promoter

    NA

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFR-mTq2-antiFRET-P2A-RCaMP1h

    Plasmid

    #191467

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    RCaMP1h
    

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

    Plasmid

    #194725

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D908A

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFR-mScarletI-antiFRET-P2A-EGFP

    Plasmid

    #191458

    Purpose

    Compare the intensity of EGFP to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    EGFP
    

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge