We narrowed to 9,391 results for: bli

1. eSpCas9(1.1)-T2A-Puro

   Plasmid

   #101039

   Purpose

   Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

   Depositor

   Andrea Németh

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3xFLAG and T2A-Puro

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLIX403-EYFP-ccdB-Puro

   Plasmid

   #158549

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROPSeq-PP7-p65-HSF

   Plasmid

   #196719

   Purpose

   CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAG-mito-dsRED2

   Plasmid

   #173071

   Purpose

   mitochondria targeted dsRED2 expressed off of CAG promoter

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mem-mCherry-pTRIP

   Plasmid

   #163521

   Purpose

   Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

   Depositor

   Thomas Weber

   Insert

   membrane targeted mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AbVec_IGHC

   Plasmid

   #183701

   Purpose

   Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.

   Depositor

   David Nemazee

   Insert

   IGHG1 (IGHG1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK647

   Plasmid

   #71701

   Purpose

   Produces E. coli thioredoxin reductase (EcTrxB)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin reductase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIX403-iRFP-ccdB-Puro

   Plasmid

   #158566

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   iRFP

   Expression

   Mammalian

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AbVec_IGKC

   Plasmid

   #183702

   Purpose

   Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.

   Depositor

   David Nemazee

   Insert

   IGKC (IGKC )
   

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD451-pA-APEX2

    Plasmid

    #157863

    Purpose

    Bacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labeling

    Depositor

    Alex Kentsis

    Insert

    pA-APEX2
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135978

    Purpose

    gRNA against the right loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA2
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Promoter

    CB

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AbVec_IGLC

    Plasmid

    #183703

    Purpose

    Encode human Ig lambda chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGLC2 (IGLC2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid

    #174893

    Purpose

    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Insert

    mCherry and EGFP
    

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG CMV Empty Puro

    Plasmid

    #237881

    Purpose

    Empty mammalian expression plasmid for cloning untagged proteins

    Depositor

    Meghan Rego, Addgene Research Program

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. 4X3G

    Plasmid

    #64663

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SIAH1 (SIAH1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    April 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. TMC1-TwinStrepII

    Plasmid

    #83267

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    TMC1 (Tmc1 Mouse)
    

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR_Hu_BIRC5(WT)

    Plasmid

    #136351

    Purpose

    Human BIRC5 cDNA in Gateway pENTR

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Gateway entry vector

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pExp-MsyB

    Plasmid

    #112570

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AbVec2.0-mIghg3

    Plasmid

    #127160

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg3 (Ighg3 Mouse)
    

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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