Skip to main content
Addgene
Showing: 261 - 280 of 471 results
  1. MARS_pNIC-CTB10H

    Plasmid
    #153053
    Purpose
    Expression construct for producing Avi tagged Methionyl-tRNA synthetase
    Depositor
    Insert
    MARS (MARS1 Human)
    Use
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. GARS_pNIC-Bio3

    Plasmid
    #153049
    Purpose
    Expression construct for producing Avi tagged Aspartyl-tRNA synthetase
    Depositor
    Insert
    GARS (GARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pZNF215-donor

    Plasmid
    #113804
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF215 with GFP
    Depositor
    Insert
    ZNF215 homology arms flanking EGFP-IRES-Neo cassette (ZNF215 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs2239733, rs2239732, hg38:11:6956348:T>G, rs1…
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pSMAD3-donor

    Plasmid
    #112311
    Purpose
    CRISPR donor plasmid to tag human transcription factor SMAD3 with GFP
    Depositor
    Insert
    SMAD3 homology arms flanking EGFP-IRES-Neo cassette (SMAD3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:15:67189762:C>T, rs11629568, rs8025774
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. INPP5EA

    Plasmid
    #42478
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    INPP5EA (INPP5E Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  7. STK3A-c042

    Plasmid
    #53622
    Purpose
    bacterial expression plasmid
    Insert
    STK3A (STK3 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. LARS_pNIC-Bio3

    Plasmid
    #153052
    Purpose
    Expression construct for producing Avi tagged Leucyl-tRNA synthetase
    Depositor
    Insert
    LARS (LARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Not full length protein
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pHOXB13-donor

    Plasmid
    #113814
    Purpose
    CRISPR donor plasmid to tag human transcription factor HOXB13 with GFP
    Depositor
    Insert
    HOXB13 homology arms flanking EGFP-IRES-Neo cassette (HOXB13 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs139887310
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. PECI

    Plasmid
    #38859
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    PECI (Eci2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  11. CMYA5tnT/pACT2

    Plasmid
    #98126
    Purpose
    CMYA5 (aa 3859 – 4069) Leu allele (4063) in a GAL4 AD vector, used for yeast-two hybrid
    Depositor
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    rs10043986 (NP_705838.3:p.Pro4063Leu)
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pZNF354B-donor

    Plasmid
    #112309
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF354B with GFP
    Depositor
    Insert
    ZNF354B homology arms flanking EGFP-IRES-Neo cassette (ZNF354B Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:5:178884427:G>A, hg38:5:178883445:C>T,…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pRS263

    Plasmid
    #122379
    Purpose
    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon
    Depositor
    Insert
    HSP90
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 261 - 280 of 471 results