We narrowed to 11,872 results for: pcDNA

1. pcDNA3.1_rSERT_C109A_Y107C

   Plasmid

   #190174

   Purpose

   For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway

   Depositor

   Gary Rudnick

   Article

   Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.

   Insert

   SLC6A4 Serotonin Transporter (Slc6a4 Rat)
   

   Use

   Tags

   FLAG, His6, and c-myc

   Expression

   Mammalian

   Mutation

   changed Cys109 to alanine, changed tyr107 to cyst…

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1_rSERT_X5C_S277C

   Plasmid

   #190177

   Purpose

   For mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibility

   Depositor

   Gary Rudnick

   Article

   Zhang et al J Biol Chem. 2006 Nov 24;281(47):36213-2

   Insert

   rat serotonin transporter (Slc6a4 Rat)
   

   Use

   Tags

   FLAG, His6, and c-myc

   Expression

   Mammalian

   Mutation

   Cys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcdna3.1_mouseST6galI

   Plasmid

   #178435

   Purpose

   murine St6gal1 expression plasmid

   Depositor

   Deborah Fass

   Article

   Ilani et al EMBO J. 2022 Oct 17:e111869. doi: 10.152

   Insert

   St6gal1 (St6gal1 Mouse)
   

   Use

   Tags

   6xHIS-TEV Cleavage Site

   Expression

   Mammalian

   Mutation

   Coding sequnce for amino acids 85-403

   Promoter

   CMV

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mApple_pcDNA3.1+

   Plasmid

   #182855

   Purpose

   Monomer expression vector

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

   Insert

   mApple
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_hNKD2

   Plasmid

   #174329

   Purpose

   encodes human NKD2 cDNA with a C-terminal MycHis Tag.

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Tags

   MycHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AlACR2_EYFP_pcDNA3.1

   Plasmid

   #161023

   Purpose

   Expresses AlACR2 in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

   Insert

   AlACR2
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AlACR3_EYFP_pcDNA3.1

   Plasmid

   #161024

   Purpose

   Expresses AlACR3 in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

   Insert

   AlACR3
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RsCCR2_EYFP_pcDNA3.1

   Plasmid

   #159125

   Purpose

   This plasmid encodes RsCCR2 rhodopsin domain

   Depositor

   John Spudich

   Article

   Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

   Insert

   RsCCR2
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RsCCR1_EYFP_pcDNA3.1

   Plasmid

   #159124

   Purpose

   This plasmid encodes RsCCR1 rhodopsin domain

   Depositor

   John Spudich

   Article

   Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

   Insert

   RsCCR1
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RaCCR2_EYFP_pcDNA3.1

    Plasmid

    #159123

    Purpose

    This plasmid encodes RaCCR2 rhodopsin domain

    Depositor

    John Spudich

    Article

    Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

    Insert

    RaCCR2
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GtCCR1_EYFP_pcDNA3.1

    Plasmid

    #159120

    Purpose

    This plasmid encodes GtCCR1 rhodopsin domain

    Depositor

    John Spudich

    Article

    Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

    Insert

    GtCCR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MvChR1_EYFP_pcDNA3.1

    Plasmid

    #159119

    Purpose

    This plasmid encodes MvChR1 rhodopsin domain

    Depositor

    John Spudich

    Article

    Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

    Insert

    MvChR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1_mutNSDHL

    Plasmid

    #136428

    Purpose

    Expresses NSDHL harboring all currently known mutations within exons 4 and 6 associated with CHILD syndrome.

    Depositor

    Antonio Amelio

    Article

    Getz et al Oral Surg Oral Med Oral Pathol Oral Radi

    Insert

    human NSDHL with mutations in exon 4 and exon 6 (NSDHL Human, Synthetic)
    

    Use

    Tags

    V5-His tag

    Expression

    Mammalian

    Mutation

    C>T = A105V; G>C = A182P; G>A = R199H; G…

    Promoter

    CMV

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5_FRT_GFP_12AAA_mCH

    Plasmid

    #127111

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    GFP-mCherry fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5_FRT_GFP_6TS_mCH

    Plasmid

    #127110

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    GFP-mCherry fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1_hE2F7mg_6ntmut

    Plasmid

    #73075

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns wi…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1_hE2F7mg_10ntmut

    Plasmid

    #73076

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns wi…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1mLCAD_K42Q_FLAG

    Plasmid

    #24487

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lys42Gln

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1mLCAD_K42A_FLAG

    Plasmid

    #24488

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lys42Ala

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3_mScarlet-LAMP1

    Plasmid

    #185138

    Purpose

    Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

    Depositor

    Andrew York

    Article

    Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

    Insert

    Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-21 of LAMP1

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge