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Showing: 521 - 560 of 847 results

  1. pUSF2-donor

    Plasmid
    #112361
    Purpose
    CRISPR donor plasmid to tag human transcription factor USF2 with GFP
    Depositor
    Kevin White
    Insert
    USF2 homology arms flanking EGFP-IRES-Neo cassette (USF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:35278400:T>A, hg38:19:35278455:G>C
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112361-plasmid-map-sequence-id-229684

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  2. pNR2F1-donor

    Plasmid
    #112388
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR2F1 with GFP
    Depositor
    Kevin White
    Insert
    NR2F1 homology arms flanking EGFP-IRES-Neo cassette (NR2F1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs974916569
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112388-plasmid-map-sequence-id-229852

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  3. pZNF250-donor

    Plasmid
    #112383
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF250 with GFP
    Depositor
    Kevin White
    Insert
    ZNF250 homology arms flanking EGFP-IRES-Neo cassette (ZNF250 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:144882271:C>G,hg38:8:144882273:T>C
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112383-plasmid-map-sequence-id-229838

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  4. pZNF140-donor

    Plasmid
    #112363
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF140 with GFP
    Depositor
    Kevin White
    Insert
    ZNF140 homology arms flanking EGFP-IRES-Neo cassette (ZNF140 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:133105834:A>G, hg38:12:133105852:A>G
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112363-plasmid-map-sequence-id-229689

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  5. pZNF639-donor

    Plasmid
    #112345
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF639 with GFP
    Depositor
    Kevin White
    Insert
    ZNF639 homology arms flanking EGFP-IRES-Neo cassette (ZNF639 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:179333542:G>T,rs978050614
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112345-plasmid-map-sequence-id-229659

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  6. pTBPL1-donor

    Plasmid
    #112316
    Purpose
    CRISPR donor plasmid to tag human transcription factor TBPL1 with GFP
    Depositor
    Kevin White
    Insert
    TBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs35776966, hg38:6:133987646:GTGTAT>-
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112316-plasmid-map-sequence-id-228480

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  7. attB53_SNCB+_attB53

    Plasmid
    #183614
    Purpose
    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183614-plasmid-map-sequence-id-361603

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  8. pCas9FnCpf1GG

    Plasmid
    #131013
    Purpose
    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131013-plasmid-map-sequence-id-257083

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  10. pCSDC2-donor

    Plasmid
    #113813
    Purpose
    CRISPR donor plasmid to tag human transcription factor CSDC2 with GFP
    Depositor
    Kevin White
    Insert
    CSDC2 homology arms flanking EGFP-IRES-Neo cassette (CSDC2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113813-plasmid-map-sequence-id-229910

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  11. pZNF215-donor

    Plasmid
    #113804
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF215 with GFP
    Depositor
    Kevin White
    Insert
    ZNF215 homology arms flanking EGFP-IRES-Neo cassette (ZNF215 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs2239733, rs2239732, hg38:11:6956348:T>G, rs1…
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113804-plasmid-map-sequence-id-231033

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  12. pZNF41-donor

    Plasmid
    #112375
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF41 with GFP
    Depositor
    Kevin White
    Insert
    ZNF41 homology arms flanking EGFP-IRES-Neo cassette (ZNF41 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:X:47447496:G>T, hg38:X:47448269:C>A, h…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112375-plasmid-map-sequence-id-229739

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  13. pELF4-donor

    Plasmid
    #112371
    Purpose
    CRISPR donor plasmid to tag human transcription factor ELF4 with GFP
    Depositor
    Kevin White
    Insert
    ELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112371-plasmid-map-sequence-id-229726

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  14. pZBTB26-donor

    Plasmid
    #112356
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB26 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB26 homology arms flanking EGFP-IRES-Neo cassette (ZBTB26 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:9:122919395:A>G, hg38:9:122919458:T>G,…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112356-plasmid-map-sequence-id-229674

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  15. pZNF84-donor

    Plasmid
    #112330
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF84 with GFP
    Depositor
    Kevin White
    Insert
    ZNF84 homology arms flanking EGFP-IRES-Neo cassette (ZNF84 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs623100, hg38:12:133058872:T>C, hg38:12:13305…
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112330-plasmid-map-sequence-id-228558

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  16. pZNF75A-donor

    Plasmid
    #112307
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF75A with GFP
    Depositor
    Kevin White
    Insert
    ZNF75A homology arms flanking EGFP-IRES-Neo cassette (ZNF75A Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:3317462:A>T, hg38:16:3317715:C>T, h…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112307-plasmid-map-sequence-id-228460

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  17. pZNF764-donor

    Plasmid
    #112298
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF764 with GFP
    Depositor
    Kevin White
    Insert
    ZNF764 homology arms flanking EGFP-IRES-Neo cassette (ZNF764 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:30555233:G>A, hg38:16:30555234:C>G,…
    Promoter
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112298-plasmid-map-sequence-id-228408

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  19. pERF-donor

    Plasmid
    #112299
    Purpose
    CRISPR donor plasmid to tag human transcription factor ERF with GFP
    Depositor
    Kevin White
    Insert
    ERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112299-plasmid-map-sequence-id-228410

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  20. pHOXB13-donor

    Plasmid
    #113814
    Purpose
    CRISPR donor plasmid to tag human transcription factor HOXB13 with GFP
    Depositor
    Kevin White
    Insert
    HOXB13 homology arms flanking EGFP-IRES-Neo cassette (HOXB13 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs139887310
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113814-plasmid-map-sequence-id-229911

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  21. pFOXO4-donor

    Plasmid
    #112312
    Purpose
    CRISPR donor plasmid to tag human transcription factor FOXO4 with GFP
    Depositor
    Kevin White
    Insert
    FOXO4 homology arms flanking EGFP-IRES-Neo cassette (FOXO4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112312-plasmid-map-sequence-id-228464

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  22. pE2F3-donor

    Plasmid
    #113809
    Purpose
    CRISPR donor plasmid to tag human transcription factor E2F3 with GFP
    Depositor
    Kevin White
    Insert
    E2F3 homology arms flanking EGFP-IRES-Neo cassette (E2F3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Unknown
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    113809-plasmid-map-sequence-id-241245

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  23. pZNF57-donor

    Plasmid
    #112296
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF57 with GFP
    Depositor
    Kevin White
    Insert
    ZNF57 homology arms flanking EGFP-IRES-Neo cassette (ZNF57 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs2288959, rs2288958, rs10410539, hg38:19:2918211…
    Promoter
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112296-plasmid-map-sequence-id-225584

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  24. pHOXC13-donor

    Plasmid
    #113827
    Purpose
    CRISPR donor plasmid to tag human transcription factor HOXC13 with GFP
    Depositor
    Kevin White
    Insert
    HOXC13 homology arms flanking EGFP-IRES-Neo cassette (HOXC13 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113827-plasmid-map-sequence-id-230204

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  25. pGLIS3-donor

    Plasmid
    #113812
    Purpose
    CRISPR donor plasmid to tag human transcription factor GLIS3 with GFP
    Depositor
    Kevin White
    Insert
    GLIS3 homology arms flanking EGFP-IRES-Neo cassette (GLIS3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs10118031, rs10973702, rs912257
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113812-plasmid-map-sequence-id-229905

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  26. pZNF211-donor

    Plasmid
    #112382
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF211 with GFP
    Depositor
    Kevin White
    Insert
    ZNF211 homology arms flanking EGFP-IRES-Neo cassette (ZNF211 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:57641976:T>C, hg38:19:57641979:A>T,…
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112382-plasmid-map-sequence-id-229835

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  27. pZNF589-donor

    Plasmid
    #112340
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF589 with GFP
    Depositor
    Kevin White
    Insert
    ZNF589 homology arms flanking EGFP-IRES-Neo cassette (ZNF589 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:48267877:T>C, hg38:3:48267890:C>T, h…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112340-plasmid-map-sequence-id-229653

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  28. pHBP1-donor

    Plasmid
    #112336
    Purpose
    CRISPR donor plasmid to tag human transcription factor HBP1 with GFP
    Depositor
    Kevin White
    Insert
    HBP1 homology arms flanking EGFP-IRES-Neo cassette (HBP1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112336-plasmid-map-sequence-id-228570

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  29. pZNF565-donor

    Plasmid
    #112315
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF565 with GFP
    Depositor
    Kevin White
    Insert
    ZNF565 homology arms flanking EGFP-IRES-Neo cassette (ZNF565 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:36182553:A>T, hg38:19:36182595:A>G,…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112315-plasmid-map-sequence-id-228476

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  30. pNR1D2-donor

    Plasmid
    #112314
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR1D2 with GFP
    Depositor
    Kevin White
    Insert
    NR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:23977483:A>T, hg38:3:23977515:A>G, h…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112314-plasmid-map-sequence-id-228473

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  31. pSMAD3-donor

    Plasmid
    #112311
    Purpose
    CRISPR donor plasmid to tag human transcription factor SMAD3 with GFP
    Depositor
    Kevin White
    Insert
    SMAD3 homology arms flanking EGFP-IRES-Neo cassette (SMAD3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:15:67189762:C>T, rs11629568, rs8025774
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112311-plasmid-map-sequence-id-228463

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  32. pCYC1pr-GNSI (LEU)

    Plasmid
    #111450
    Purpose
    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111450-plasmid-map-sequence-id-211605

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  33. pGS1222

    Plasmid
    #63791
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eGFP-Twin-Strep tag
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63791-plasmid-map-sequence-id-106478

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  36. pNM3689

    Plasmid
    #154111
    Purpose
    miniMos single component RMCE landing site vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Insert
    loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
    Use
    Minimos
    Tags
    Expression
    Mutation
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154111-plasmid-map-sequence-id-306070

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  37. pNM3749

    Plasmid
    #154108
    Purpose
    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    Use
    Cre/Lox; Rmce
    Tags
    Expression
    Worm
    Mutation
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154108-plasmid-map-sequence-id-306067

    Enlarge

  38. pBALU1

    Plasmid
    #69316
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp
    Depositor
    Oliver Hobert
    Article
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    GFPint-FgF
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    69316-plasmid-map-sequence-id-266105

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  39. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

    Enlarge

  40. pATF4-donor

    Plasmid
    #113805
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP
    Depositor
    Kevin White
    Insert
    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs140428747
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113805-plasmid-map-sequence-id-231035

    Enlarge

Showing: 521 - 560 of 847 results