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pHAGE2-TetOminiCMV-OKM

Plasmid

#136554

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00320

Plasmid

#34445

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6376 (E2f1 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pOTF3-AK (clone3)

Plasmid

#21165

Depositor

James Thomson

Article

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

POUF5F1 (genomic DNA) (POU5F1 Human)

Use

Cre/Lox; Homologous recombination

Tags

Expression

Mutation

NeoR, EGFP, and SV40 polyA are between the two ge…

Promoter

Available since

June 25, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Use

Tags

Expression

Mutation

Deleted the partial 3'ribozyme sequence in T…

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp3-myc

Plasmid

#11813

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 3 (CASP3 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-mMALAT1_3' Sense

Plasmid

#60647

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-bGH pA Sense

Plasmid

#60645

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-SV40 pA Sense

Plasmid

#60643

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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insulator_seq_UENU_donor

Plasmid

#221459

Purpose

Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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KHBD00716

Plasmid

#39744

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17228 (pros Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pCDEF3‐hTIM3 (no tag)

Plasmid

#49212

Purpose

expresses human Tim3

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (HAVCR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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KHBD00050

Plasmid

#34212

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11502 (svp Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-OSKM

Plasmid

#136541

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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Ai-LV (CS-TRE-mOKS-PRE-Ubc-rtTA-I2G)

Plasmid

#198058

Purpose

Generation of rodent iPS cells from somatic cells

Depositor

Hiromitsu Nakauchi

Article

Yamaguchi et al Methods Mol Biol. 2014;1210:143-50. doi:

Insert

mOct4, mKlf4, mSox2 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

Plasmid

#105036

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

Plasmid

#105035

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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SP-GFP-CD274-UTR

Plasmid

#121142

Purpose

Mammalian expression of GFP tagged CD274 with its own 3'UTR

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

CD274 with its own 3'UTR (CD274 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP_C1 I3

Plasmid

#61397

Purpose

Expresses GFP-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pmCherry_C1 I3

Plasmid

#61398

Purpose

Expresses mCherry-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3

Plasmid

#61401

Purpose

Expresses GFP-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only