We narrowed to 426 results for: 41594

1. hprtSAGFP

   Plasmid

   #41594

   Purpose

   In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

   Depositor

   Maria Jasin

   Article

   Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

   Insert

   SA-GFP reporter
   

   Use

   Mouse Targeting; Dsb repair reporter substrate

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. WT_IFL_G1A-T2A-A28C_R

   Plasmid

   #200185

   Purpose

   WT PA14 I-F Leader and G1A-T2A-A28C Repeat

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G1A-T2A-A28C in the I-F repeat

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRAW-457

   Plasmid

   #200213

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Use

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas1 H25A mutation

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRAW-458

   Plasmid

   #200214

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Use

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas1 E184A mutation

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRAW-459

   Plasmid

   #200215

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Use

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas1 K214A mutation

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRAW-460

   Plasmid

   #200216

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Use

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas2 R55E-N56D mutations

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRAW-461

   Plasmid

   #200217

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Use

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas2 K11D-R12E mutations

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRAW-462

   Plasmid

   #200218

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Use

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas2 K11D-R12E, R55E-N56D mutations

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCherry-NIP45 wt

   Plasmid

   #210266

   Purpose

   Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Insert

   NIP45 wt siRES (NFATC2IP Synthetic)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRES

   Promoter

   CMV/TetO2

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-NIP45 wt

   Plasmid

   #210262

   Purpose

   Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Insert

   NIP45 wt siRES (NFATC2IP Synthetic)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   siRES

   Promoter

   CMV/TetO2

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PRPF4-1

    Plasmid

    #156158

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF4 (PRPF4 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SFRS10

    Plasmid

    #156007

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SFRS10 (TRA2B Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RBM8A

    Plasmid

    #155910

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    RBM8A (RBM8A Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SFRS2IP

    Plasmid

    #156012

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SFRS2IP (SCAF11 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SFRS17A

    Plasmid

    #156010

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SFRS17A (SFRS17A Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid

    #179527

    Purpose

    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 K233A/K234A/K253A/K263A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed lysines 233, 234, 253, and 263 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)

    Plasmid

    #155136

    Purpose

    Expresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    D243G/L244G/F245G/M247G/S248G/M249G/A250G

    Promoter

    tac

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFPc3 K-RAS 4B

    Plasmid

    #236452

    Purpose

    transient overexpression of K-RAS 4B in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    K-RAS 4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFB1.HMBP.PrS.MTF2

    Plasmid

    #125169

    Purpose

    Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    MTF2 (MTF2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CNOT3

    Plasmid

    #155467

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CNOT3 (CNOT3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

    Plasmid

    #233629

    Purpose

    Expression of bicistronic reporter with mutated TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    AGAAGG in TOP motif

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge