We narrowed to 436 results for: 41594

1. hprtSAGFP

   Plasmid

   #41594

   Purpose

   In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

   Depositor

   Maria Jasin

   Article

   Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

   Insert

   SA-GFP reporter
   

   Use

   Mouse Targeting; Dsb repair reporter substrate

   Expression

   Mammalian

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pCon-IFl_Con-IIAr

   Plasmid

   #192212

   Purpose

   Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-F Leader and Consensus II-A Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCon-IEl_Con-IEr

   Plasmid

   #192213

   Purpose

   Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-E Leader and Consensus I-E Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCon-ICl_Con-ICr

   Plasmid

   #192214

   Purpose

   Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-C Leader and Consensus I-C Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCon-IIAl_Con-IIAr

   Plasmid

   #192215

   Purpose

   Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus II-A Leader and Consensus II-A Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX1-IFl_Con-IFr

   Plasmid

   #192216

   Purpose

   Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   I-F leader and I-F repeat construct not studied further in this paper (X1)
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX2-IFl_Con-IFr

   Plasmid

   #192217

   Purpose

   Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   I-F leader and I-F repeat construct not studied further in this paper (X2)
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. WT_IFL_G1A-T2A-A28C_R

   Plasmid

   #200185

   Purpose

   WT PA14 I-F Leader and G1A-T2A-A28C Repeat

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
   

   Expression

   Bacterial

   Mutation

   G1A-T2A-A28C in the I-F repeat

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRAW-457

   Plasmid

   #200213

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas1 H25A mutation

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRAW-458

   Plasmid

   #200214

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas1 E184A mutation

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRAW-459

    Plasmid

    #200215

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas1 K214A mutation

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRAW-460

    Plasmid

    #200216

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 R55E-N56D mutations

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRAW-461

    Plasmid

    #200217

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E mutations

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRAW-462

    Plasmid

    #200218

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E, R55E-N56D mutations

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-NIP45 wt

    Plasmid

    #210266

    Purpose

    Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 wt siRES (NFATC2IP Synthetic)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRES

    Promoter

    CMV/TetO2

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFP-NIP45 wt

    Plasmid

    #210262

    Purpose

    Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 wt siRES (NFATC2IP Synthetic)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    siRES

    Promoter

    CMV/TetO2

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PRPF4-1

    Plasmid

    #156158

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF4 (PRPF4 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RBM8A

    Plasmid

    #155910

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    RBM8A (RBM8A Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SFRS2IP

    Plasmid

    #156012

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SFRS2IP (SCAF11 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SFRS17A

    Plasmid

    #156010

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SFRS17A (SFRS17A Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid

    #179527

    Purpose

    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 K233A/K234A/K253A/K263A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed lysines 233, 234, 253, and 263 to alanine

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge