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HsCERK

Plasmid

#118600

Purpose

Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate

Depositor

Kevin Lynch

Article

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens ceramide kinase

Tags

FLAG

Expression

Yeast

Promoter

GAL1, 10

Available Since

Feb. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-mdlC

Plasmid

#134590

Purpose

Low Phosphate inducible mdlC benzylformate decarboxylase

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

mdlC (benzylformate decarboxylase) C -terminal his tag

Tags

6x-His

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoBp-GFPuv

Plasmid

#139073

Purpose

Low phosphate inducible promoter phoBp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoBp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-ugpBp-GFPuv

Plasmid

#139078

Purpose

Low phosphate inducible promoter ugpBp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

ugpBp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Nnmt in situ probe

Plasmid

#45631

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Nnmt in situ probe (Nnmt Mouse)

Use

In situ

Mutation

fragment contains bp# 454-908 of NM_010924.2

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pSMART-HCKan-yibDp-VHH72

Plasmid

#202467

Purpose

Expresses the nanobody VHH72 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-H6antivenin

Plasmid

#202470

Purpose

Expresses the nanobody H6 antivenin after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody H6 antivenin

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-1B5

Plasmid

#202472

Purpose

Expresses the nanobody 1B5 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 1B5

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-2E7

Plasmid

#202473

Purpose

Expresses the nanobody 2E7 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 2E7

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-3E3

Plasmid

#202474

Purpose

Expresses the nanobody 3E3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 3E3

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-C9BoNT-A

Plasmid

#202475

Purpose

Expresses the nanobody C9 BoNT-A after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-E7BoNT-A

Plasmid

#202476

Purpose

Expresses the nanobody E7 BoNT-A after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody E7 BoNT/A

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-JM3

Plasmid

#202477

Purpose

Expresses the nanobody JM3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody JM3

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-J3

Plasmid

#202478

Purpose

Expresses the nanobody J3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody J3

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-m36.4

Plasmid

#202479

Purpose

Expresses the nanobody m36.4 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody m36.4

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-Re5D06

Plasmid

#202480

Purpose

Expresses the nanobody Re5D06 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody Re5D06

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-Re9F06

Plasmid

#202481

Purpose

Expresses the nanobody Re9F06 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody Re9F06

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-ppiB

Plasmid

#202482

Purpose

Expresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

peptidyl-prolyl cis-trans isomerase (ppiB)

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCASCADE-LCAmp-trxB

Plasmid

#202465

Purpose

Expresses a guide RNA to silence trxB after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

trxB guide RNA

Expression

Bacterial

Promoter

ugpB

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLT237

Plasmid

#174300

Purpose

Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

PolC homology arm

Expression

Bacterial

Mutation

changed Cysteine 669 to Tyrosine

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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