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pJJB716

Plasmid

#218600

Purpose

Express Pex27

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex27

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1335

Plasmid

#218607

Purpose

Express Pex28

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex28

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1502

Plasmid

#218609

Purpose

Express Pex29

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex29

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB715

Plasmid

#218599

Purpose

Express Pex25

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex25

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1578

Plasmid

#218620

Purpose

Express Pex22, Pex6, Pex15, and Pex8

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-APEX3

Plasmid

#202590

Purpose

Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

Depositor

Reuben Harris

Article

Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

Insert

mNeonGreen-APEX3-V5 (APX1 )

Use

Retroviral

Tags

V5 epitope and mNeonGreen

Expression

Mammalian

Mutation

APEX2 L242A

Promoter

MESV LTR

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGbH500-Nanog-AXP

Plasmid

#140279

Purpose

a targeting plasmid for Nanog-APEX

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

endogenous

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBlue-loxPuro-APEX-PML_LASA

Plasmid

#140273

Purpose

a targeting plasmid for APEX-PML

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

endogenous

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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SOPP3-HA-nes

Plasmid

#229071

Purpose

Expresses SOPP3 in the cytosol of mammalian cells

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express SOPP3 in the cytosol of mammalian cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

Plasmid

#182824

Purpose

Cre-dependent expression of cytosolic APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

APEX.NES-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-AP-SpyCatcher

Plasmid

#223636

Purpose

Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Use

Tags

His10, AP fragment of split APEX2 tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP5

Plasmid

#160088

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Use

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTXB1-TP3

Plasmid

#160086

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Use

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

Plasmid

#117687

Purpose

U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

Depositor

Erik Sontheimer

Article

Gao et al Methods Enzymol. 2019;616:365-383. doi:

Inserts

Kanamycin cassette
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

Plasmid

#182825

Purpose

Cre-dependent expression of H2B-APEX fusion

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

H2B.APEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS887_pTetR-P2A-BFPnls/sgAlpha

Plasmid

#108651

Purpose

Alpha satellite repeats targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Alpha satellite repeats targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

Plasmid

#182826

Purpose

Cre-dependent expression of membrane localized APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

LckAPEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTXB1-TP4

Plasmid

#160087

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Use

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge