We narrowed to 3,338 results for: ubc

1. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

   Plasmid

   #170578

   Purpose

   Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDKL5 (CDKL5 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   E854G- please see depositor comments

   Promoter

   Ubiquitin

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUGW-UBC-EGFP-2A-BoNT-B (LC)

   Plasmid

   #185722

   Purpose

   Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   botulinum neurotoxin type B light chain
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-15b-UbcH5b/2419

   Plasmid

   #11475

   Depositor

   Allan Weissman

   Article

   Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

   Insert

   UbcH5b (UBE2D2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   n/a

   Promoter

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

4. p663-UBC-V5-TurboID-V5-STOP_IDG-K

   Plasmid

   #158159

   Purpose

   Gateway destination clone of TurboID-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-TurboID-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p667-UBC-VRK3-V5-miniTurbo_IDG-K

   Plasmid

   #189902

   Purpose

   Gateway destination clone of VRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   VRK3 (VRK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p663-UBC-miniTurbo-V5-CDK14_IDG-K

   Plasmid

   #158172

   Purpose

   Gateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK14 (CDK14 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p663-UBC-TurboID-V5-BRSK2_IDG-K

   Plasmid

   #135272

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TurboID-V5-BRSK2 (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p667-UBC-CDK12-V5_IDG-K

   Plasmid

   #135245

   Purpose

   Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK12-V5 (CDK12 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psubCAG-WPRE-hVEGF-D-Strept

   Plasmid

   #119227

   Purpose

   Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.

   Depositor

   Kari Alitalo

   Insert

   VEGFD (VEGFD Human)
   

   Use

   AAV

   Tags

   Strept

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p667-UBC-GFP-V5-TurboID_IDG-K

    Plasmid

    #135241

    Purpose

    Gateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    GFP-V5-TurboID
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p663-UBC-miniTurbo-V5-RIOK1_IDG-K

    Plasmid

    #214464

    Purpose

    Gateway destination clone of RIOK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-RIOK1 (RIOK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. UbC NLS-HA-MCP-YFP

    Plasmid

    #31230

    Depositor

    Robert Singer

    Article

    Grunwald et al Nature. 2010 Sep 30. 467(7315):604-7.

    Insert

    NLS-HA-MCP-YFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-UBC9-K14R-A129K

    Plasmid

    #228948

    Purpose

    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

    Depositor

    Marcin Suskiewicz

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGHHHHHHSSG…

    Expression

    Bacterial

    Mutation

    changed Lysine 14 to Arginine, changed Alanine 12…

    Promoter

    T7-lac

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. p663-UBC-V5-miniTurbo-V5-STOP_IDG-K

    Plasmid

    #158158

    Purpose

    Gateway destination clone of miniTurbo-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-miniTurbo-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pESC-URA-GFP-Ubc9ts

    Plasmid

    #20369

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Y68L This mutation renders Ubc9 temperature-sens…

    Promoter

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

16. p663-UBC-miniTurbo-V5-MAPK15_IDG-K

    Plasmid

    #170581

    Purpose

    Gateway destination clone of MAPK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MAPK15 (MAPK15 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p667-UBC-BCKDK-V5-miniTurbo_IDG-K

    Plasmid

    #158189

    Purpose

    Gateway destination clone of BCKDK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    BCKDK-V5-miniTurbo (BCKDK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCR3-FLAG-Ubc13-C87A

    Plasmid

    #43939

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    ubiquitin-conjugating enzyme E2N (UBE2N Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    C87A

    Promoter

    Available Since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p663-UBC-miniTurbo-V5-CDK11A_IDG-K

    Plasmid

    #214461

    Purpose

    Gateway destination clone of CDK11A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK11A (CDK11A Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p667-UBC-ADCK5-V5-miniTurbo_IDG-K

    Plasmid

    #189909

    Purpose

    Gateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ADCK5 (ADCK5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge