We narrowed to 2,954 results for: ER

1. EGFP-ST-P2A-pSC-CAAX

   Plasmid

   #207637

   Purpose

   A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC-cUnaG-LPETG

   Plasmid

   #207635

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-Best1-Txnip(C247S_LL351-352AA)

   Plasmid

   #206344

   Purpose

   AAV plasmid expressing mutant TXNIP in retinal pigment epithelium

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

   Insert

   Txnip (Txnip Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   C247S, L351A, L352A

   Promoter

   human Best1

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-dora[B]_rescue_construct

   Plasmid

   #190638

   Purpose

   Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   3xHA

   Expression

   Insect

   Mutation

   Changed threonine 295 to methionine

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-dora[A]_rescue_construct

   Plasmid

   #190640

   Purpose

   Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   3xHA

   Expression

   Insect

   Mutation

   Truncated to contain amino acids 1-945

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRTpsiCHECK_SEAP

   Plasmid

   #154461

   Purpose

   Expression of SEAP and firefly luciferase

   Depositor

   Markus Landthaler

   Article

   Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

   Insert

   SEAP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CTAG_eGFP-GHtag-HDEL

   Plasmid

   #136096

   Purpose

   FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJS697

   Plasmid

   #168778

   Purpose

   Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Type

   Empty backbone

   Use

   Tags

   Cmyc and HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ad-HisFlag-USP19ER

   Plasmid

   #155244

   Purpose

   for generating adenoviruses expressing the ER isoform of rat USP19

   Depositor

   Simon Wing

   Article

   Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

   Insert

   USP19 (ER isoform) (Usp19 Rat)
   

   Use

   Adenoviral

   Tags

   6XHis-3XFlag

   Expression

   Mutation

   H118R- please see dep. comments

   Promoter

   CMV

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-HisFlag-rUSP19ER-CAmut

    Plasmid

    #155237

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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