Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,360 results for: CAG

Showing: 4171 - 4200 of 9360 results

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Bxb1-GA_AIO-TetOn_ISL1-LHX3_Down-Tandem

Plasmid

#241395

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ISL1 and LHX3

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-ISL1-LHX3; rtTA-T2A-mTagBFP2 (ISL1, LHX3 Human)

Tags

NLS

Expression

Mammalian

Promoter

TRE; CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1022

Plasmid

#242503

Purpose

Expression of ZZ-3xTEV-HsATG14 and ZZ-3xTEV-HsBECN1 in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Inserts

ATG14 (ATG14 Human)
BECN1 (BECN1 Human)

Tags

ZZ-3xTEV

Expression

Mammalian

Promoter

CAG and CMV

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHLsec hSOD1

Plasmid

#232481

Purpose

vector for transient transfection expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

Depositor

Lukas Trantirek

Article

Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

Insert

Homo sapiens superoxide dismutase 1 (SOD1 Human)

Tags

DYKDDDDK (FLAG-tag)

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHLsec-dasTMPRSS2

Plasmid

#207850

Purpose

Expresses directed activation strategy TMPRSS2 in mammalian cells, such as HEK293F- or CHO-cells

Depositor

Ingrid De Meester

Article

Verhulst et al J Extracell Vesicles. 2025 Mar;14(3):e70

Insert

transmembrane serine protease 2 (TMPRSS2 Human)

Tags

AviTag, 8xHIS tag

Expression

Mammalian

Mutation

deleted amino acid 1-108, S251D R252D Q253D S254D…

Promoter

CAG

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 E484Q (CAA)

Plasmid

#212423

Purpose

Encodes SARS-CoV-2 spike E484Q for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484Q (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484Q

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 deltaE156-F157+R158G (GGA)

Plasmid

#212421

Purpose

Encodes SARS-CoV-2 spike E156-F157deletion,R158G for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E156-F157deletion,R158G (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E156-F157deletion,R158G

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L981F (TTC)

Plasmid

#212486

Purpose

Encodes SARS-CoV-2 spike L981F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L981F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L981F

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T572I (ATC)

Plasmid

#212404

Purpose

Encodes SARS-CoV-2 spike T572I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T572I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T572I

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (Yellowhammer)

Plasmid

#212402

Purpose

Encodes SARS-CoV-2 spike L5F,D614G,Q677H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L5F,D614G,Q677H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L5F,D614G,Q677H

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T29I (ATA)

Plasmid

#212403

Purpose

Encodes SARS-CoV-2 spike T29I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T29I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T29I

Promoter

CAG

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 V1104L (TTA)

Plasmid

#223274

Purpose

Encodes SARS-CoV-2 spike V1104L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike V1104L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

V1104L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Y453F (TTC)

Plasmid

#212369

Purpose

Encodes SARS-CoV-2 spike Y453F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Y453F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Y453F

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.5

Plasmid

#212505

Purpose

Encodes SARS-CoV-2 variant BA.5 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.5 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F456L (CTT)

Plasmid

#212542

Purpose

Encodes SARS-CoV-2 Spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (CTT) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 FL.1.5.1

Plasmid

#212539

Purpose

Encodes SARS-CoV-2 variant FL1.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike FL.1.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRP.ABCA4 c.5461-10T

Plasmid

#226553

Purpose

Minigene encoding the ABCA4 c.5461-10T mutation

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

ABCA4 (ABCA4 Human)

Expression

Mammalian

Mutation

c.5461-10T intronic mutation

Promoter

CAG

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q183H (CAT)

Plasmid

#223279

Purpose

Encodes SARS-CoV-2 spike Q183H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q183H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Q183H

Promoter

CAG

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q498R (CGA)

Plasmid

#212475

Purpose

Encodes SARS-CoV-2 spike Q498R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q498R (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Q498R

Promoter

CAG

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 A67V (GTT)

Plasmid

#212395

Purpose

Encodes SARS-CoV-2 spike A67V for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike A67V (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

A67V

Promoter

CAG

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N969K (AAA)

Plasmid

#212485

Purpose

Encodes SARS-CoV-2 spike N969K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N969K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N969K

Promoter

CAG

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F456L (TTA)

Plasmid

#212536

Purpose

Encodes SARS-CoV-2 spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q52H (CAT)

Plasmid

#212535

Purpose

Encodes SARS-CoV-2 spike Q52H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q52H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Q52H

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 P1143L (CTC)

Plasmid

#212571

Purpose

Encodes SARS-CoV-2 spike P1143L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike P1143L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

P1143L

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 G446S (AGC)

Plasmid

#212558

Purpose

Encodes SARS-CoV-2 spike G446S for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike G446S (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

G446S

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T859N (AAC)

Plasmid

#212447

Purpose

Encodes SARS-CoV-2 spike T859N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T859N (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T859N

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 G446S (AGC)

Plasmid

#212468

Purpose

Encodes SARS-CoV-2 spike G446S for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike G446S (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

G446S

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 G75V (GTA) T76I (ATT)

Plasmid

#212443

Purpose

Encodes SARS-CoV-2 spike G75V, T76I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike G75V, T76I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

G75V, T76I

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 V70F (TTT)

Plasmid

#212431

Purpose

Encodes SARS-CoV-2 spike V70F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike V70F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

V70F

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F888L (CTT)

Plasmid

#212397

Purpose

Encodes SARS-CoV-2 spike F888L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F888L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F888L

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 H655Y (TAT)

Plasmid

#212364

Purpose

Encodes SARS-CoV-2 spike H655Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike H655Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

H655Y

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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