We narrowed to 6,062 results for: cat.2

1. pKESaPar

   Plasmid

   #211829

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCsRNAP

   Plasmid

   #182746

   Purpose

   Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Insert

   small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBridge-tyrosinase.σ2

   Plasmid

   #197415

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-2 σ2
   

   Use

   Tags

   GAL4-DNA binding domain fragment, HA tag, and nuc…

   Expression

   Yeast

   Mutation

   encodes R42G substitution, contains silent substi…

   Promoter

   ADH1 and MET25

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHIV-EGFP.Flag-EZH2-mt2*

   Plasmid

   #220246

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2* (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB1.HMBP.PrS.EZH2-mt2

   Plasmid

   #220240

   Purpose

   Expression of an EZH2 mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   Polyhedrin

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p1.2-GS

   Plasmid

   #162770

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-N-Myc_535-1102 USP7

   Plasmid

   #131246

   Purpose

   Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses USP7 amino acids 535-1102. This truncat…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-N-Myc_1-560 USP7

   Plasmid

   #131244

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses USP7 amino acids 1 - 560. This truncati…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIV-EGFP.Flag-EZH2-mt2

   Plasmid

   #220245

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSKA397

    Plasmid

    #80380

    Purpose

    mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    cat
    metE
    

    Use

    Synthetic Biology; Mini-tn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cat promoter and cpcG2Δ59

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS327

    Plasmid

    #51787

    Purpose

    multicopy (YEp) vector with a LYS2 marker and a 2-μm origin of replication

    Depositor

    David Stillman

    Article

    Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. M-PKD2 (OF2-3)

    Plasmid

    #21370

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type II (PKD2 Human)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    wild-type*

    Promoter

    Available since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. 915-Plakophilin 2a-Flag

    Plasmid

    #32230

    Depositor

    Kathleen Green

    Article

    Chen et al J Biol Chem. 2002 Mar 22;277(12):10512-2

    Insert

    Plakophilin 2a (PKP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY2F

    Plasmid

    #53285

    Purpose

    Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    lcyE (LUT2 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. YCpLac111

    Plasmid

    #53249

    Purpose

    contains a single replication origin (ARS1) used for plasmid replication assay

    Depositor

    Jean Cook

    Article

    Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGFP-IBGFTet

    Plasmid

    #33356

    Depositor

    Doug Mortlock

    Article

    Chandler et al Dev Biol. 2009 Mar 15;327(2):590-602. Ep

    Insert

    EGFP-IRES-Bgeo + TetR
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. PA-TNXL/pcDNA3

    Plasmid

    #51994

    Purpose

    mammalian expression of a 'caged' Ca2+ indicator for fluorescent imaging at specific locations and time points

    Depositor

    Takeharu Nagai

    Article

    Matsuda et al Sci Rep. 2013;3:1398. doi: 10.1038/srep0

    Insert

    PA-TNXL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pUR-sap1+

    Plasmid

    #15984

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Genetics. 2007 Feb . 175(2):553-66.

    Insert

    Sap1 (sap1 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pk335-CARGO-6mer-44kb-DSF

    Plasmid

    #227494

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 44kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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