Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,639 results for: WUS

Showing: 5601 - 5640 of 6639 results

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CMV5 N-HA hHIF1a P402A

Plasmid

#246216

Purpose

Used for transfection of cells for expression N-HA hHIF1a P402A

Depositor

Sheng-cai Lin

Article

Zhang et al Cell Res. 2015 Sep;25(9):1025-42. doi: 1

Insert

HIF1a (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

P402A

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-ePiv (CJT553)

Plasmid

#248215

Purpose

ePiv invertase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

ePiv invertase

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

ePiv(E257R, E285R, T290R)

Promoter

CMV and T7

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-IS621 (CJT489)

Plasmid

#248213

Purpose

bpNLS-tagged IS621 bridge recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-IS621

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-Bxb1 (EMK569)

Plasmid

#248208

Purpose

Bxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Bxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -EGFP

Plasmid

#246994

Purpose

Can be used to generate AAV virus that will express EGFP from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

EGFP

Use

AAV

Promoter

EF1α

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -hKCNJ2-HA tag

Plasmid

#246995

Purpose

Can be used to generate AAV virus that will express human KCNJ2 with Ala115 HA tag from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

hKCNJ2 (KCNJ2 Human)

Use

AAV

Tags

HA tag (Internal at Ala115)

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -hKCNJ2-V77E-HA tag

Plasmid

#246996

Purpose

Can be used to generate AAV virus that will express human KCNJ2 V77E mutant with Ala115 HA tag from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

KCNJ2 (KCNJ2 Human)

Use

AAV

Tags

HA tag (Internal at Ala115)

Mutation

V77E

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -hKCNJ2

Plasmid

#246998

Purpose

Can be used to generate AAV virus that will express human KCNJ2 from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

hKCNJ2 (KCNJ2 Human)

Use

AAV

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)-FLEx(loxP)-eYFP(nuclear)-2A-N2cG

Plasmid

#248664

Purpose

Helper virus for monosynaptic tracing; Expresses nuclear eYFP and Rabies Glycoprotein (G) of CVS-N2c strain in Cre-expressing cells under the CAG promoter.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Inserts

N2c(G)
nuc-eYFP

Use

AAV

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)--FLEx(loxP)-splitTVA:HA

Plasmid

#248665

Purpose

Helper virus for monosynaptic tracing; Expresses TVA and an HA tag in Cre-expressing cells under the CAG promoter. To be co-injected with Rabies Glycoprotein (G) viruses.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

TVA

Use

AAV

Tags

HA

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-FDX1

Plasmid

#251696

Purpose

Gateway-adapted FDX1 ORF

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

FDX1 ferredoxin 1 (FDX1 Human)

Use

Gateway Cloning

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR221-SLC31A1

Plasmid

#251694

Purpose

Gateway-adapted SLC31A1 ORF

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

SLC31A1 solute carrier family 31 member 1 (SLC31A1 Human)

Use

Gateway Cloning

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR221-BCL2

Plasmid

#251695

Purpose

Gateway-adapted BCL2 ORF

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

BCL2 BCL2 apoptosis regulator (BCL2 Human)

Use

Gateway Cloning

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GSDMD-Flag WT

Plasmid

#249726

Purpose

Express GSDMD WT in mammalian cells

Depositor

Hao Wu

Article

Du et al Nature. 2024 Jun;630(8016):437-446. doi:

Insert

gasdermin D (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pTarget-entry

Plasmid

#220996

Purpose

Prokaryotic expression of mRFP and GFP. Target sequences are inserted in place of the RFP gene through AatII/KpnI restriction ligation

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

mRFP, GFP

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN4-DDX3X-IDR1+DDX6-ΔIDR

Plasmid

#240013

Purpose

Expresses human DDX6-DDX3X-IDR1+DDX6-ΔIDR fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX3X-IDR1+DDX6-ΔIDR

Use

Lentiviral

Tags

3X Flag

Mutation

TTAAAAAAAGATAACATC

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pEvol-chKlacRS-IPYE

Plasmid

#232160

Purpose

A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

Depositor

Nanxi Wang

Article

Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

Insert

chKlacRS-IPYE

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLPS-pCDH-DDX3X-IDR1+DDX6-ΔIDR

Plasmid

#240018

Purpose

Expresses human DDX3X-IDR1+DDX6-ΔIDR fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX3X-IDR1+DDX6-ΔIDR

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PSIN4-3_Flag-ATP5MG

Plasmid

#232951

Purpose

Expresses ATP5MG

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

ATP5MG (ATP5MG Human)

Use

Lentiviral

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-3_Flag-ATP5MF

Plasmid

#232950

Purpose

Expresses ATP5MF

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

ATP5MF (ATP5MF Human)

Use

Lentiviral

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-3_Flag-ATP5PB

Plasmid

#232949

Purpose

Expresses ATP5PB

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

ATP5PB (ATP5F1 Human)

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-7

Plasmid

#228989

Purpose

For bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-7

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-9

Plasmid

#228968

Purpose

For bacterial expression of anti-GFP nanobody LaG94-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-12

Plasmid

#228970

Purpose

For bacterial expression of anti-GFP nanobody LaG94-12, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-12

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-3

Plasmid

#228963

Purpose

For bacterial expression of anti-GFP nanobody LaG94-3, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-3

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-8

Plasmid

#228967

Purpose

For bacterial expression of anti-GFP nanobody LaG94-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-6

Plasmid

#228966

Purpose

For bacterial expression of anti-GFP nanobody LaG94-6, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-6

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-18

Plasmid

#228972

Purpose

For bacterial expression of anti-GFP nanobody LaG94-18, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-18

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-2

Plasmid

#228975

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaGIgG-4

Plasmid

#228998

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-4, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaGIgG-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-2

Plasmid

#228962

Purpose

For bacterial expression of anti-GFP nanobody LaG94-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-1

Plasmid

#228961

Purpose

For bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-9

Plasmid

#228993

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-8

Plasmid

#228992

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-10

Plasmid

#228978

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-4

Plasmid

#228964

Purpose

For bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-5

Plasmid

#228987

Purpose

For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-6

Plasmid

#228988

Purpose

For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-6

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-8

Plasmid

#228976

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-9

Plasmid

#228977

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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