We narrowed to 75,825 results for: Rest

1. pLenti-LmoCas7

   Plasmid

   #126489

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas7
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-LmoCas5

   Plasmid

   #126490

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas5
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-LmoCas6

   Plasmid

   #126491

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFBD-Akt1(T308A/S473A)

   Plasmid

   #86578

   Purpose

   Expresses human full-length Akt1(T308A/S473A) phosphomutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   T308A/S473A phosphomutant

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBabe_puro_DEST_Flag_PRKCI

   Plasmid

   #45263

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   PKCI (PRKCI Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pMY-MCS-GSG-eGFP

   Plasmid

   #163350

   Purpose

   Retroviral backbone for in-frame cloning of a C-terminally GFP labeled gene of interest.

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR-FRB:Akt1(D274A)

   Plasmid

   #86634

   Purpose

   Expresses FRB-tagged human Akt1(D274A) kinase-inactive mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   FRB-Akt1(D274A) (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lyn-CFP-GAI(1-172)

   Plasmid

   #37313

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Use

   Tags

   CFP and Lyn

   Expression

   Mammalian

   Mutation

   contains aa 1-172

   Promoter

   CMV

   Available since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pmCherry-PH1(K14A)

   Plasmid

   #86600

   Purpose

   Expresses mCherry-tagged PH(K14A) domain of human Akt1

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   K14A mutant of Akt1 PH domain (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid

    #181858

    Purpose

    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-EKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lyn-CFP-GAI(1-532)

    Plasmid

    #37314

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Use

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-532

    Promoter

    CMV

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pBabe_puro_DEST_Flag_TLOC1 (220aa)

    Plasmid

    #45273

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pFBD-Akt1(K14A)

    Plasmid

    #86562

    Purpose

    Expresses human full-length Akt1(K14A) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    K14A mutation in the PH domain

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lyn-CFP-GAI(1-151)

    Plasmid

    #37312

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Use

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-151

    Promoter

    CMV

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCSP1-mp53AS

    Plasmid

    #20896

    Depositor

    Stefano Piccolo

    Article

    Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

    Insert

    p53AS (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' natural EcoRV and SacI restriction sites …

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

16. EC71139

    Plasmid

    #154062

    Purpose

    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    pZmUbi
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BpiI restriction sites were r…

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid

    #82469

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mRNA capping enzyme (Rngtt Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    changed Lysine 294 to Alanine

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO-myc NES-mCE NLS-Flag

    Plasmid

    #82468

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Myc-NES-mCE-Flag (Rngtt Mouse, HIV)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-LmoCascade-IL1RNcrRNA

    Plasmid

    #126493

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo IL1RN cr03
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-LmoCascade-HBE1crRNA

    Plasmid

    #126494

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo HBE1 cr06
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge