We narrowed to 4,060 results for: gast

1. pUASp YFP Ras85D DN

   Plasmid

   #52246

   Purpose

   Drosophila expression of YFP-Ras85D dominant negative mutant

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   S17N; DN mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. FLAG-MED29

   Plasmid

   #15409

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

   Insert

   MED29 (MED29 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

3. K401-Micro

   Plasmid

   #122485

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Matt Thomson

   Article

   Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

   Insert

   His tag MBP Kinesin 1-401 SspB micro
   

   Tags

   H6-MBP-TEV and SspB micro

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-401

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-6P-2_dCrk

   Plasmid

   #131146

   Purpose

   Expresses GST::dCrk (based on crk isoform RA).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. OA-1045C

   Plasmid

   #125005

   Purpose

   Expresses gRNAs targeting hid, eve, and wingless

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUASTattB_NSnoFbox-vhhGFP4

   Plasmid

   #35578

   Depositor

   Markus Affolter

   Article

   Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

   Insert

   NSnoFbox-vhhGFP4 (slmb Fly)
   

   Expression

   Insect

   Mutation

   F box domain of slmb deleted.

   Available Since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAFMW-Nbr-D435A/E437A

   Plasmid

   #40829

   Purpose

   Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

   Depositor

   Phillip Zamore

   Article

   Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

   Insert

   Nbr D435A/E437A (Nbr Fly)
   

   Tags

   3xFLAG and 6xMyc

   Expression

   Insect

   Mutation

   D435A/E437A, part of the DEDD motif; mutations p…

   Promoter

   Actin5C

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pT7-MS2-HA-C1-MBP-DmBam_13-36_AG

   Plasmid

   #148839

   Purpose

   Mammalian Expression of DmBam_13-36

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

   Insert

   DmBam_13-36 (bam Fly)
   

   Expression

   Mammalian

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUAST-SIK3 T196E

   Plasmid

   #52975

   Purpose

   insect expression of SIK3 T196E constitutively active mutant

   Depositor

   Marc Montminy, Biao Wang

   Article

   Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

   Insert

   SIK3 (Sik3 Fly)
   

   Tags

   HA

   Expression

   Insect

   Mutation

   T196E constitutively active mutant

   Promoter

   Hsp70

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

10. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149426

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[Sxl, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. RCAS(A) dRhoA V14 (CT#846)

    Plasmid

    #13949

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

    Insert

    RhoA (Rho1 Fly)
    

    Use

    Retroviral; Avian expression

    Mutation

    G14V mutation

    Available Since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pUAST-SIK3 T196A

    Plasmid

    #52974

    Purpose

    insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    T196A LKB1 phosphorylation defective mutant

    Promoter

    Hsp70

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pUAST-SIK3 K70M

    Plasmid

    #52973

    Purpose

    insect expression of SIK3 K70M (kinase dead mutant)

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    K70M kinase dead mutant

    Promoter

    Hsp70

    Available Since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

14. FLAG-MED6

    Plasmid

    #15411

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED6 (Med6 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. Ths pUAST-HA

    Plasmid

    #69769

    Purpose

    Thisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    Klingseisen et al Development. 2009 Jul;136(14):2393-402.

    Insert

    Thisbe (FGF8like-1)
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA-Tag

    Expression

    Insect

    Mutation

    Encodes amino acids 1-739 of Thisbe (NP_610701.2)…

    Promoter

    hsp70 promoter

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

16. KHBD00041

    Plasmid

    #34203

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG18455 (Optix Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pET19b-MED17

    Plasmid

    #15412

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED17 (MED17 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pUASTLOTattB_mCherry-CD8-vhhGFP

    Plasmid

    #163930

    Purpose

    Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. BII-gR-PtW-eSpCas9

    Plasmid

    #133357

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Tags

    HA-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. LN-entry

    Plasmid

    #128012

    Purpose

    Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    LN-3xFlag
    

    Use

    Lamdan entry vector

    Tags

    LN-3xFlag

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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