We narrowed to 12,677 results for: sequence

1. CROPseq-Puro-12xMS2

   Plasmid

   #212628

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPseq-Puro-4xMS2

   Plasmid

   #212626

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL475

   Plasmid

   #219503

   Purpose

   Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL7

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAT_CD28ICD

   Plasmid

   #197098

   Purpose

   This plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

   Depositor

   Wendell Lim

   Article

   Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

   Insert

   This plasmid contains the coding sequence for the intracellular domain of CD28.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGG-GTS-TurboID

   Plasmid

   #209397

   Purpose

   Transiently expressing TurboID fused with a Golgi targeting sequence (GTS) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   GTS-3XHA-TurboID
   

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR2_IRdist_scram

   Plasmid

   #162319

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The distal Upstream Motif site is scrambled

   Promoter

   pBAD

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)

   Plasmid

   #193050

   Purpose

   Encodes guide RNA expression targeting PX740 landing pad

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   Guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pASPIre4

   Plasmid

   #196656

   Purpose

   Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

   Insert

   Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SpeI site in Bxb1 CDS

   Promoter

   rhamnose-inducible promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TEG +Q111T

   Plasmid

   #187435

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   changed glutamine at 117 to threonine

   Promoter

   PH and p10

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEM.F02R

    Plasmid

    #198662

    Purpose

    Easy-MISE toolkit pEM-plasmid containing GFP coding sequence preceded by a linker sequence with HI protruding ends

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    linker+GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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