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pCDZ2FE_Champ1_1-812
Plasmid#105675PurposeExpresses Flag-tagged full-length human Champ1 proteinDepositorInsertChamp1 (CHAMP1 Human)
UseTags2X Flag tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
raft-APEX2
Plasmid#205089PurposeExpresses lipid-raft targeted APEX2 for proximity proteomics. Vector: pcDNA™4/TO. Insert: lipid raft target sequence (MGCVCSS) + mCherry +V5 (GKPIPNPLLGLDST) +APEX2DepositorInsertAPEX2 (raft-mCherry-V5 tagged)
UseTagslipid raft targeting sequence (MGCVCSS), mCherry,…ExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV Synapsin Intron H2B Gcamp 6s wpre Pzac2.1
Plasmid#74150Purposecalcium sensorDepositorInsertGCaMP6s
UseAAVTagsH2BExpressionMutationPromoterSynapsinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSD_N-SpCas9-1-573-FRB-1xNLS
Plasmid#107298PurposeExpresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cellsDepositorInsertTruncated SpCas9 (aa 1-573) fused to FRB
UseCRISPRTagsFRB and SV40 NLSExpressionMammalianMutationTruncated SpCas9 (aa 1-573)PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET28a SV40 ST full length
Plasmid#132636Purposeexpresses SV40 ST full length in E. coliDepositorInsertSV40 small t antigen
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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LIMK1
Plasmid#39181PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertLIMK1 (LIMK1 Human)
UseBaculovirus expressionTagsHis TevExpressionMutationPromoterpolyhedrinAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-SELP0K
Plasmid#67007PurposeExpresses malE-SELP0K in E. coliDepositorInsertmalE-SELP0K
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
p415PGK1-AtMAX1(pYL759)
Plasmid#178289PurposeExpresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5DepositorInsertAtMAX1
UseTagsExpressionYeastMutationPromoterPGK1Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
K560_AviN_coBirA
Plasmid#129766PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DPP4
Plasmid#158439PurposeGateway Cloning compatible entry vector for the human DPP4 gene.DepositorInsertDPP4 (DPP4 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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3CEG
Plasmid#60107PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBIRC6:SDC136-F10:C41228 (BIRC6 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
psd44-G13GA
Plasmid#46827Purposeexpression of mutant G protein alpha 13 in mammalian cellsDepositorInsertGNA13 (GNA13 Human)
UseLentiviralTagsExpressionMammalianMutationG225APromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CI
Plasmid#51565PurposeExpression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcI
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22-6His-SUMO-Nsp10
Plasmid#154960PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA_DiCre2_59.F2
Plasmid#60209PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Bacteriophage P1, Human, Synthetic)
UseTagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 19-59 o…PromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Nras61K-Puro
Plasmid#49404PurposeMSCVpuro based vector, expressing Nras mutant 61K.DepositorInsertN Ras K61 (NRAS Human)
UseRetroviralTagsExpressionMammalianMutation61K constitutively activatedPromoterMSCV promotorAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
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CAPZB2-TwinStrepII
Plasmid#83194PurposeExpression of tagged protein in mammalian cellsDepositorInsertCAPZB2 (Capzb Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
4L59
Plasmid#60087PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3:JMC057-A08:C223215 (L3MBTL3 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti.puro.MCV.ER.RAZ.1
Plasmid#114381PurposeThis lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=330 aa.DepositorInsertMCV NCCR and tumor T antigen regions
UseLentiviralTagsExpressionMammalianMutationPromoterMCV NCCR (viral own promoter)Available sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-FLAG-hSRF (#799)
Plasmid#78345PurposeDoxycycline-inducible lentiviral overexpression of human SRF ORF with a FLAG tagDepositorInsertSRF (SRF Human)
UseLentiviralTagsFLAGExpressionMutationPromoterTRE2Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Aiolos AA130-189 in Artichoke
Plasmid#74451PurposeMonitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorInsertAiolos (IKZF3) AA130-189 (IKZF3 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Beta2-PAmcherry1
Plasmid#114190Purposestudying clustering of Beta2 receptors by superresolution microscopy (PALM)DepositorInsertAdrenergic receptor beta 2 (ADRB2 Human)
UseTags3HA, FLAG, and PAmcherry1ExpressionMammalianMutationPromoterSV40Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-BRT17
Plasmid#67000PurposeExpresses malE-BRT17 in E. coliDepositorInsertmalE-BRT17
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Delta (JCE594)
Plasmid#182025Purposeyeast expression of mouse PKC Delta with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Delta (Prkcd Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1
Plasmid#184294PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1DepositorInsertsgRNA-Adrb1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3
Plasmid#107736PurposeIn the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.DepositorInsertsynaptophysin-GCaMP6s, mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoternEFAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA
Plasmid#139513PurposeB6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy numberDepositorInsertCAG rox stop rox td-sfGFP dre reporter
UseMouse Targeting; Dre/roxTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetO-Kir2.1-IRES-TLZ
Plasmid#32642DepositorInserttetO-Kir2.1-IRES-TLZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
