We narrowed to 31,919 results for: Eng

1. pRGEB32-CrActin-35S

   Plasmid

   #226708

   Purpose

   For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.

   Depositor

   Fenglin Deng

   Article

   Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

   Insert

   CrActin
   

   Use

   CRISPR

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRGEB32-CrActin-UBI

   Plasmid

   #226709

   Purpose

   For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

   Depositor

   Fenglin Deng

   Article

   Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

   Insert

   CrActin
   

   Use

   CRISPR

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRGEB32-CrU6-2-UBI

   Plasmid

   #226707

   Purpose

   For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

   Depositor

   Fenglin Deng

   Article

   Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

   Insert

   CrU6-2
   

   Use

   CRISPR

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS

   Plasmid

   #194751

   Purpose

   To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2

   Depositor

   Chaoyang Li

   Article

   Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

   Type

   Empty backbone

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. eNS2B47NS3 S135A

   Plasmid

   #155318

   Purpose

   DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   S135A

   Promoter

   M13

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pW34

   Plasmid

   #158210

   Purpose

   LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.

   Depositor

   Yasuo Yoshikuni

   Article

   Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

   Insert

   LuxABCDE
   

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pM-ABE

   Plasmid

   #196292

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding ABE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pS-sg:GFP

   Plasmid

   #196296

   Purpose

   35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM-Cas9

   Plasmid

   #196325

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding SpCas9 in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213973

    Purpose

    AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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