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pSL0341 (pTarget)

Plasmid

#200906

Purpose

Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

pTarget

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pChlamy/Cre

Plasmid

#205440

Purpose

An expression vector for Cre recombinase (codon usage for C. reinhardtii)

Depositor

Masamichi Kamihira

Article

Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

Insert

Cre recombinase gene

Use

Chlamydomonas expression

Promoter

Heat shock protein 70A (HSP70A)/ribulose bisphosp…

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2783 (pTnsC-VP64_Vch)

Plasmid

#200896

Purpose

Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsC-VP64

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2669 (pTnsABf_Vch)

Plasmid

#200897

Purpose

Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsA-TnsB

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSL0915 (CRISPR with target 3)

Plasmid

#130643

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(target-3)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pConRef-2H12

Plasmid

#221182

Purpose

cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

Plasmid

#207031

Purpose

Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

mRuby3

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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Incoherent feedforward (IFF) plasmid

Plasmid

#169748

Purpose

Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV-tetO2

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-YTHDF2

Plasmid

#204406

Purpose

E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

YTHDF2 (YTHDF2 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2621 (pCas8_Vch)

Plasmid

#200893

Purpose

Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas8

Tags

NLS

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2623 (pCas6_Vch)

Plasmid

#200895

Purpose

Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas6

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-SARS-CoV-2-Nucleocapsid

Plasmid

#204403

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2622 (pCas7_Vch)

Plasmid

#200894

Purpose

Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas7

Tags

NLS

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSL0426 (CRISPR with spc3)

Plasmid

#130642

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(spacer-3)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMIG-Flag-RIPK1-wt

Plasmid

#102677

Purpose

retroviral expression plasmid for RIPK1-wt

Depositor

Hasam Habelhah

Article

Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

Insert

RIPK1 (RIPK1 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Promoter

unknown

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pJK503

Plasmid

#155380

Purpose

dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Available Since

May 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-Flag-TRAF5

Plasmid

#102679

Purpose

retroviral expression plasmid for TRAF5

Depositor

Hasam Habelhah

Article

Zhang et al J Mol Biol. 2009 Jun 12;389(3):495-510.

Insert

TRAF5 (Traf5 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBCC155-MXMT

Plasmid

#79816

Purpose

CFP-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

CC155/CFP (155–239)

Expression

Plant

Promoter

CaMV 35S promoter and CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBGC155-MXMT

Plasmid

#79818

Purpose

GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

GC155/GFP (155–239)

Expression

Plant

Promoter

CaMV 35S promoter and CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mm

Plasmid

#51059

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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