We narrowed to 9,414 results for: virus

1. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTC217

   Plasmid

   #70018

   Purpose

   Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

   Depositor

   Daniel Voytas

   Article

   Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

   Insert

   Nuclease (Cas9/sgRNA) + Donor + GVR
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Use

   Tags

   C-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE2-SpG

   Plasmid

   #159978

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpG
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac HT JS-Munc18c

   Plasmid

   #135555

   Purpose

   Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18c
   

   Use

   Tags

   6xHis tag and TEV site

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Spike-VLP

   Plasmid

   #173922

   Purpose

   Expresses virus-like particles based upon the original SARS-Cov-2

   Depositor

   Andreas Stuermer

   Inserts

   SARS-2 Spike Protein (S SARS-COV-2, Synthetic, Human)
   SARS-Cov-2 E protein (E SARS-COV-2, Synthetic, Human)
   SARS-Cov-2 M Protein (M SARS-COV-2, Synthetic, Human)
   

   Use

   Tags

   no tags, native protein

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

   Available since

   April 11, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEV53D

   Plasmid

   #44168

   Depositor

   John Olsen

   Article

   Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

   Insert

   Equine infectious anemia virus (EIAV) gag-pol,rev, and S2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A131T mutation in gag; Deletion of viral LTR sequ…

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. LRG (Lenti_sgRNA_EFS_GFP)

   Plasmid

   #65656

   Purpose

   Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter-driven sgRNA expression and EFS promo…

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEXPqcxip-hSURF4-FLAG

   Plasmid

   #159139

   Purpose

   Hu SURF4 -flag mammalian expression Gateway vector.

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   surfeit 4 (SURF4 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (cytomegalovirus) enhancer and the MSV (mouse…

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE2-SpRY

    Plasmid

    #159979

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpRY
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE2-NG

    Plasmid

    #159977

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-NG
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Use

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-FLEX-rev-ChR2-tdtomato

    Plasmid

    #18917

    Purpose

    Cre-dependent viral expression of ChR2-tdTomato for neuronal excitation

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    Channelrhodopsin 2-tdtomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    tdtomato

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PE2-VQR

    Plasmid

    #159982

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-VQR
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-NP-V5-B/BRISBANE/60/2008

    Plasmid

    #187923

    Purpose

    Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-FLEX-rev-ChR2(H134R)-mCherry

    Plasmid

    #18916

    Purpose

    Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    mCherry

    Expression

    Mutation

    H134R

    Promoter

    CAG

    Available since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PE2-VRER

    Plasmid

    #159984

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-VRER
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CVS-N2c-nl.EGFP-SypGFP

    Plasmid

    #172380

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized EGFP + SypEGFP
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PE2-VRQR

    Plasmid

    #159983

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-VRQR
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-PA-B/BRISBANE/60/2008

    Plasmid

    #187920

    Purpose

    Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase acidic protein
    

    Use

    Tags

    UNTAGGED

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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