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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,919 results for: Eng

Showing: 1431 - 1440 of 31919 results

  1. pM-Cas12a

    Plasmid
    #196291
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    Use
    CRISPR
    Tags
    Flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196291-plasmid-map-sequence-id-389824

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  2. pS-cr:GFP

    Plasmid
    #196295
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    SpeI-DR-BsaI-DR-SpeI
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196295-plasmid-map-sequence-id-390035

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  4. Td-CBEmax

    Plasmid
    #196600
    Purpose
    Mammalian expression of Td-CBEmax
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196600-plasmid-map-sequence-id-386793

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  5. eNS2B47NS3 L30SF31S

    Plasmid
    #155319
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    L30 to S, F31 to S
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155319-plasmid-map-sequence-id-311251

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  7. pTK Cas9-2A-Citrine

    Plasmid
    #92393
    Purpose
    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    Cas9 2A Citrine
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    thymidine kinase promoter
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92393-plasmid-map-sequence-id-190866

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Showing: 1431 - 1440 of 31919 results