We narrowed to 144,768 results for: ins

1. pQC

   Plasmid

   #221134

   Purpose

   Contains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminator

   Depositor

   Daniel Daley

   Article

   Cumming et al ACS Synth Biol. 2024 Apr 27. doi: 10.102

   Inserts

   [PibpA-gfpASV]
   [PcpxPmcherryASV] OPT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cpxP and ibpA

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBad-PhoCl2f

   Plasmid

   #164036

   Purpose

   Improved PhoCl variant with faster dissociation rate

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl2f
   

   Use

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBad promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Rab5DN(S34N)

   Plasmid

   #35141

   Depositor

   Sergio Grinstein

   Article

   Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

   Insert

   Rab5a (RAB5A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Dominant Negative- S34N

   Promoter

   CMV

   Available since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

4. AICSDP-117:POLR2A-mEGFP

   Plasmid

   #164500

   Purpose

   Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

   Depositor

   Allen Institute for Cell Science

   Insert

   POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLSL

   Plasmid

   #51493

   Purpose

   T-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Type

   Empty backbone

   Use

   Plant t-dna plasmid

   Tags

   Expression

   Mutation

   Promoter

   virion-sense LIR and 2x35S

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPomyces-SaCas9

   Plasmid

   #129553

   Purpose

   Contains codon-optimized SaCas9 and gRNA for streptomyces

   Depositor

   Mingzi M. Zhang, Fong Tian Wong

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   SaCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHelper_TetR/pTet-Tnpase (carbR)

   Plasmid

   #213911

   Purpose

   ATc-inducible himar1C9 transposase expression - carb/amp resistance

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   TetR/ptet ATc-inducible himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXF276

   Plasmid

   #139048

   Purpose

   MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   lov domain (includes STOP codon)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSDH52

   Plasmid

   #139049

   Purpose

   MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   epdz (includes STOP codon)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDD363

    Plasmid

    #91829

    Purpose

    LoxP-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    LoxP-SEC-LoxP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. DHFR-myc

    Plasmid

    #20214

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. p40PX-EYFP

    Plasmid

    #19011

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p40phox (NCF4 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    aa1-148 (PX domains)

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-p40PX(aa1-148)

    Plasmid

    #19008

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p40phox (NCF4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa1-148 (PX domains)

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pJL1 eforRed

    Plasmid

    #106320

    Purpose

    Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    eforRed
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.GFA104.PI.eGFP.WPRE.bGH

    Plasmid

    #100896

    Purpose

    AAV expression of EGFP in astrocytes

    Depositor

    Philip Haydon

    Article

    Ortinski et al Nat Neurosci. 2010 May;13(5):584-91. doi

    Has Service

    AAV5

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFA104 (GFAP promoter)

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDD364

    Plasmid

    #91831

    Purpose

    Lox511I-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox511I-SEC-Lox511I
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDD382

    Plasmid

    #91830

    Purpose

    Lox2272-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox2272-SEC-Lox2272
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTM1_L_ZEBOV

    Plasmid

    #69120

    Purpose

    Encodes for Ebola Virus Zaire L under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    L ZEBOV (L )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PGRP-SA

    Plasmid

    #21055

    Depositor

    Johann Deisenhofer

    Article

    Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

    Insert

    PGRP-SA (PGRP-SA Fly)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-K-CAAX

    Plasmid

    #41644

    Purpose

    Expresses wild-type Dronpa fused to the membrane-anchoring K-Ras C-terminal farnesylation motif (CAAX box)

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145K-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAG-HA-rat Nrxn3beta AS4(-)

    Plasmid

    #58269

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn3beta AS4(-) (Nrxn3 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EcMBP165-cpGFP.PPYF.T203V.pRSET

    Plasmid

    #33373

    Purpose

    a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

    Depositor

    Loren Looger

    Article

    Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

    Insert

    Improved MBP based Maltose Biosensor
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted between AA1…

    Promoter

    T7

    Available since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. PKC beta II CAT

    Plasmid

    #16384

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC beta II (Prkcb Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CAT: catalytic domain, aa 329-673

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQCXIH-CDKN2A

    Plasmid

    #37104

    Depositor

    Joseph Nevins

    Article

    Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

    Insert

    CDKN2A (CDKN2A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pDD121

    Plasmid

    #91833

    Purpose

    Cas9 expression in C. elegans

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR4-HA-myc-nuc (w557-1)

    Plasmid

    #20025

    Purpose

    Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    HA, Myc, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAC-EsaR

    Plasmid

    #47643

    Purpose

    wt esaR gene under control of Plac in pACYC184

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    Plac-esaR-terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

28. EcMBP165-cpGFP.PPYF.pRSET

    Plasmid

    #33372

    Purpose

    a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

    Depositor

    Loren Looger

    Article

    Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

    Insert

    MBP based Maltose Biosensor
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted between AA1…

    Promoter

    T7

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mTB3-Myc tag

    Plasmid

    #210205

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    Myc-tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBacDual with PA+Histag

    Plasmid

    #84870

    Purpose

    Baculovirus Expression of Bacillus anthracis protective antigen

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Insert

    PA
    

    Use

    Baculovirus

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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