We narrowed to 5,891 results for: dna plasmids

1. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

   Plasmid

   #229800

   Purpose

   Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   hEF1a and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLP-sfgfp

   Plasmid

   #209994

   Purpose

   Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   sfGFP
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPT1

   Plasmid

   #209995

   Purpose

   Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   Terminator (TB1006)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPS1

   Plasmid

   #209992

   Purpose

   Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   RBS (RB0034)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL0_DE_mTurquoise2

   Plasmid

   #202155

   Purpose

   Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   mTurquoise2 gene
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. HySp5-227

   Plasmid

   #192996

   Purpose

   Plasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domain

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Sp5
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   no SP box and DNA binding domain

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. HySp5-337

   Plasmid

   #192994

   Purpose

   Plasmid that encodes for Hydra Sp5, lacking the DNA binding domain

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Sp5
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   no DNA binding domain

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL.103

   Plasmid

   #184988

   Purpose

   Express Cas9-P2A-Eco1RT

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-P2A-Eco1RT
   

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCL.027

   Plasmid

   #184964

   Purpose

   Express -Eco1 RT and ncRNA in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(wt), a1/a2 length: 12
   

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal7

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCL.102

    Plasmid

    #184987

    Purpose

    Express -Eco1 RT-P2A-Cas9

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-P2A-Cas9
    

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCL.006

    Plasmid

    #184970

    Purpose

    Express -Eco1 RT in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Integrating inducible cassette: Eco1 RT
    

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCL.005

    Plasmid

    #184971

    Purpose

    Express Cas9 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Integrating inducible cassette: Cas9
    

    Tags

    SV40NLS

    Expression

    Yeast

    Promoter

    Gal1-10

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOD_003

    Plasmid

    #155362

    Purpose

    Zeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

    Plasmid

    #107300

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107299

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. rs210-major

    Plasmid

    #113377

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 major allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2104047 major allele
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. rs210-minor

    Plasmid

    #113378

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 minor allele (G)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2104047 minor allele
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. rs438-major

    Plasmid

    #113369

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 major allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs4384382 major allele
    

    Use

    Luciferase

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

19. rs344-minor

    Plasmid

    #113366

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs34466261 minor allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs34466261 minor allele
    

    Use

    Luciferase

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. rs438-minor

    Plasmid

    #113370

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 minor allele (G)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs4384382 minor allele
    

    Use

    Luciferase

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge