We narrowed to 7,762 results for: loxP

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pAC150-KEIMA-REEP5

Plasmid

#201928

Purpose

PiggyBac Vector expressing KEIMA-REEP5

Depositor

Wade Harper

Publication

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

REEP5 (REEP5 Human)

Expression

Mammalian

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_CRF1.0

Plasmid

#208661

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

Depositor

Yulong Li

Publication

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Use

AAV

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

Plasmid

#197885

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Publication

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-DIO-VARNAM2-Kv2.1PR

Plasmid

#195533

Purpose

Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Publication

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-GCaMP6s

Plasmid

#183809

Purpose

This plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

Depositor

William Wisden

Publication

Yu et al Science. 2022 Jul;377(6601):63-72. doi:

Insert

AAV transgene TetO promoter-DIO/flex-GCaMP6

Use

AAV, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p2attB-FST344-HA-CPL

Plasmid

#131460

Purpose

Encodes human Follistatin protein

Depositor

Michele Calos

Publication

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Follistatin (FST Human)

Tags

HA

Expression

Bacterial and Mammalian

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-EGFP

Plasmid

#176274

Purpose

Viral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Publication

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-SEO-CaMKII-TVA-P2A-N2cG

Plasmid

#172363

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinase

Depositor

Yoav Ben-Simon, Peter Jonas

Publication

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CaMKIIa

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EPHB4-2A-EGFP

Plasmid

#139968

Purpose

Donor vector to knockin EGFP (separated by 2A) into EPHB4 allele

Depositor

James Thomson

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human EPHB4 knockin homology arms (EPHB4 Human)

Expression

Mammalian

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal80-3xP3-RFP

Plasmid

#127562

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal80

Use

CRISPR

Expression

Insect

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-N2H-F2-empty vector

Plasmid

#125552

Purpose

N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)

Depositor

Marc Vidal

Publication

Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

Type

Empty backbone

Use

In vitro expression

Tags

NanoLuc fragment2 alone without any attached prot…

Expression

Mammalian and Yeast

Promoter

synthetic promoter containing yeast, mammalian, a…

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLENTI_RPS10

Plasmid

#127129

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K24R- please see depositor comments

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a8

Plasmid

#124848

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-STAT3-linker-TagGFP2

Plasmid

#121427

Purpose

WT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.

Depositor

Ovijit Chaudhuri, Stanley Qi

Publication

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

STAT3-linker-TagGFP2

Tags

TagGFP2

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF13_P2A_Hygro_Barcode

Plasmid

#120499

Purpose

Barcoded lentiviral vector to express KLF13 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF13 (KLF13 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX3_P2A_Hygro_Barcode

Plasmid

#120481

Purpose

Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROG1_P2A_Hygro_Barcode

Plasmid

#120467

Purpose

Barcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROG1 (NEUROG1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EF1a_NEUROG3_P2A_Hygro_Barcode

Plasmid

#120468

Purpose

Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA11_P2A_Hygro_Barcode

Plasmid

#120453

Purpose

Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA11 (HOXA11 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-SNAPf-BICD2N(1-400)

Plasmid

#111857

Purpose

N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Publication

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICD2N(1-400)

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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