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pSIN-TRE-SspB-mCh-p60

Plasmid

#190170

Purpose

Doxycycline-inducible lentivirus vector to express SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

SspBmicro-mCherry-p60

Use

Lentiviral

Tags

SspBmicro-mCherry

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xP3-RFP

Plasmid

#130276

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

3xP3-RFP

Use

CRISPR

Expression

Insect

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_1

Plasmid

#127554

Purpose

Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 1

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCFD3-frame_selector_0

Plasmid

#127553

Purpose

Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 0

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-sgTel

Plasmid

#122659

Purpose

Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.884

Plasmid

#108536

Purpose

AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

AcuRAM + PAcu-YFP

Expression

Bacterial

Mutation

AcuR: E80K R154H L202M

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.683

Plasmid

#108531

Purpose

BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

BetIAM + PBetI-YFP

Expression

Bacterial

Mutation

BetI: S115I

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM4723:TpCC_Urease

Plasmid

#85982

Purpose

Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

Depositor

Thomas Mock

Publication

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Inserts

Cas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2

Use

CRISPR; Diatom (t. pseudonana)

Tags

NLS and YFP

Promoter

FCP and U6

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pYHY1

Plasmid

#215926

Purpose

E. coli-Bacteroides shuttle vector.

Depositor

Shannon Sirk

Publication

Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

Type

Empty backbone

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Expression

Bacterial

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5 mSynCAM3

Plasmid

#115498

Purpose

expression of mouse full-length SynCAM 3

Depositor

Thomas Biederer

Publication

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm3 (Cadm3 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pID2.02

Plasmid

#26955

Depositor

Geraldine Seydoux

Publication

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’

Expression

Worm

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pRC/CMV

Plasmid

#8514

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

HOXA9 (Hoxa9 Mouse)

Tags

FLAG and His

Expression

Mammalian

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pME Actin Vhh mCherry (JDW 1240)

Plasmid

#224499

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mCherry

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-DreERT2 (JDW 1344)

Plasmid

#224523

Purpose

A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1b-ST-WPRE

Plasmid

#214967

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

Depositor

Peng Zou

Publication

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1b-ST

Use

AAV

Promoter

human Synapsin

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-zeoCV

Plasmid

#73890

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

zeoCV

Expression

Bacterial, Mammalian, and Y…

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Top7 V48V

Plasmid

#12393

Depositor

David Baker

Publication

Kuhlman et al Science. 2003 Nov 21. 302(5649):1364-8.

Insert

Top7 V48V

Tags

6xHis

Expression

Bacterial

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
373 pTol2-fli1ep:CherryDest

Plasmid

#73493

Purpose

Tol2 vector with fli1ep element upstream of mCherry, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:mCherry-attR1-attR2

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC57-Rluc-BoxB-A114-N40

Plasmid

#50562

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Publication

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-BoxB-A114-N40

Use

Cloning vector

Promoter

lac

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFA6a-12Pk-LEU2MX6

Plasmid

#49179

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

12Pk

Expression

Yeast

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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