We narrowed to 11,272 results for: VARS

1. pRSETB/mStayGold

   Plasmid

   #212017

   Purpose

   mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   mStayGold
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pArk313

   Plasmid

   #200257

   Purpose

   Ark313 rep/cap

   Depositor

   Justin Eyquem

   Article

   Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

   Insert

   Ark313 Rep/Cap
   

   Use

   AAV

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-DUET-1-mRhubarb719-HO

   Plasmid

   #141200

   Purpose

   Expresses mRhubarb719 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb719
   heme oxygenase
   

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

   Promoter

   T7

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. piggyBAC-H2B mCherry-XL (R125K)

   Plasmid

   #189926

   Purpose

   Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

   Insert

   mCherry-XL (R125K)
   

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   differs from mCherry by R125K, W143S, I161V, Q163…

   Promoter

   CMV7

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MP6-SlugABEN-738

   Plasmid

   #214348

   Purpose

   Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
   

   Expression

   Bacterial

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA GSK3 beta wt pcDNA3

   Plasmid

   #14753

   Purpose

   Mammalian expression of human GSK3 beta with HA tag

   Depositor

   Jim Woodgett

   Article

   He et al Nature. 1995 Apr 13. 374(6523):617-22.

   Insert

   GSK3 beta (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Lenti-PEAR-mCherry

   Plasmid

   #228892

   Purpose

   All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Insert

   EGFP-IRES-mScarlet
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LT3GEPIR

   Plasmid

   #111177

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. eSrtA(4S-9) pET29b

   Plasmid

   #75146

   Purpose

   reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

   Depositor

   David Liu

   Article

   Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   reprogrammed sortase A variant
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N98D S102C A104V A118T F122A K134R F144L I182V E1…

   Promoter

   T7

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-Trono-EFS-Blast-P2A-TurboRFP

    Plasmid

    #228893

    Purpose

    Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-Tubulin-6

    Plasmid

    #56450

    Purpose

    Localization: Microtubules, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCAGGS_VP35_MARV

    Plasmid

    #135471

    Purpose

    Encodes Marburg virus VP35 gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

    Insert

    VP35
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Dsup pLVX-Puro

    Plasmid

    #180635

    Purpose

    Expresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio tolerance

    Depositor

    Christopher Mason

    Article

    Westover et al bioRxiv 2020.11.10.373571

    Insert

    Dsup
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. UBC plasmid

    Plasmid

    #169746

    Purpose

    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SaKKH-ABEmax

    Plasmid

    #119815

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJC175e

    Plasmid

    #79219

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PPSP gIII, luxAB
    

    Expression

    Bacterial

    Promoter

    PPSP

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p21-Reporter

    Plasmid

    #52432

    Purpose

    DNA damage inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

    Insert

    p21-p53 Tandem Response Element Promoter
    

    Tags

    TurboRFP

    Expression

    Mammalian

    Promoter

    13X repeated p21-p53 response element

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. VRQR-ABEmax

    Plasmid

    #119811

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcGFP1-N1-CAHS3

    Plasmid

    #90031

    Purpose

    To express GFP-tagged CAHS3 in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS3
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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