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BSP179

Plasmid

#110915

Purpose

For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Type

Empty backbone

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFH79

Plasmid

#125818

Purpose

Level0 A3A-PBE, wheat codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

A3A-PBE, wheat codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pFH68

Plasmid

#128188

Purpose

Level1 ZmUBIp::SpCas9-NG (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9-NG (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPTK012-3a-Inulinase-αMFΔ

Plasmid

#84971

Purpose

Type 3a, Inulinase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Inulinase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFPmem pA

Plasmid

#80823

Purpose

CMVmin-promoted GFPmem with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

3E-CMV-GFPCAAXpA spacer

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFH59

Plasmid

#128185

Purpose

Level1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH81

Plasmid

#128192

Purpose

Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::ScCas9 (wheat codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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YCe1911 HC_Kan_Insulator_FB_p2

Plasmid

#100686

Purpose

part designed to occupy position 22 of EMMA. Functional category: Insulator

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Insulator_FB

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pR6Ko2_FRTkanFRT

Plasmid

#243905

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aph

Expression

Bacterial

Available Since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko2_FRTcamFRT

Plasmid

#243904

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cat

Expression

Bacterial

Available Since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko2_FRTtetFRT

Plasmid

#243903

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetA

Expression

Bacterial

Available Since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko2_FRTampFRT

Plasmid

#243906

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

bla

Expression

Bacterial

Available Since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa2_FRTtetFRT

Plasmid

#243842

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetA

Expression

Bacterial

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Ptet

Plasmid

#243925

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, not including ribosome binding site. Lacks repressor, TetR.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PtetA without tetR

Expression

Bacterial

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa1_FRTkanFRT

Plasmid

#243835

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aph

Expression

Bacterial

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Plac

Plasmid

#243921

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including ribosome binding site. Lacks repressor, LacI.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PlacZYA without lacI

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Para

Plasmid

#243923

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, not including ribosome binding site. Lacks activator protein, AraC.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

ParaBAD without araC

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PR

Plasmid

#243927

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site. Lacks repressor, cI857.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PR without cI857

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Plac

Plasmid

#243910

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome binding site. Lacks repressor, LacI.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PlacZYA without lacI

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Para

Plasmid

#243912

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site. Lacks activator protein, AraC.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

ParaBAD without araC

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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